Reactivity | MuSpecies Glossary |
Applications | Flow, B/N, CyTOF-ready, ICC/IF |
Clone | 698423 |
Clonality | Monoclonal |
Host | Rat |
Conjugate | Unconjugated |
Conjugate | Catalog # | Availability | Size | Price |
---|---|---|---|---|
Alexa Fluor 350 | FAB6130U-100UG | |||
Alexa Fluor 405 | FAB6130V-100UG | |||
Alexa Fluor 488 | FAB6130G | |||
Alexa Fluor 594 | FAB6130T-100UG | |||
Alexa Fluor 647 | FAB6130R-100UG | |||
Alexa Fluor 700 | FAB6130N-100UG | |||
Alexa Fluor 750 | FAB6130S-100UG | |||
Allophycocyanin | FAB6130A | |||
Phycoerythrin | FAB6130P | |||
Specificity | Detects mouse GM-CSF R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) GM-CSF R alpha or rhGM-CSF R beta is observed. |
Source | N/A |
Isotype | IgG2a |
Clonality | Monoclonal |
Host | Rat |
Gene | CSF2RA |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Publications |
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Storage | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Reconstitution Instructions | Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration. |
Granulocyte macrophage colony stimulating factor receptor alpha (GM-CSF R alpha ), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM-CSF. GM-CSF promotes the differentiation and mobilization of granulocyte-macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effector functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1-4). Mature mouse GM-CSF R alpha is an 80 kDa type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains and a juxtamembrane WSXWS motif, a 21 aa transmembrane segment, and a 40 aa cytoplasmic domain (5). Within the ECD, mouse GM-CSF R alpha shares approximately 33% and 58% aa sequence identity with human and rat GM-CSF R alpha , respectively. Soluble forms of the human receptor retain the ability to bind GM-CSF (6, 7). GM-CSF R alpha is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non‑hematopoietic solid tumor cells (8). GM-CSF R alpha associates with the common beta chain/CD131 ( beta c), a 135 kDa transmembrane protein that is also the signal transducing component of the receptors for IL-3 and IL-5 (9, 10). Association with beta c converts GM-CSF R alpha from a low affinity to a high affinity receptor for GM-CSF (9-11). The shared usage of beta c underlies the synergism between GM-CSF, IL-3, and IL-5 in their effects on myeloid cell differentiation and activation (1, 2).
Secondary Antibodies |
Isotype Controls |
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