J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse GM-CSF Ra APC-conjugated Monoclonal Antibody (Catalog # FAB6130A, filled histogram) or isotype control antibody (Catalog # ...read more
GM-CSF-Producing ILCs in Inflamed Joints(A) Flow cytometry analysis of GM-CSF expression by CD4+ T cells and CD4− cells among CD45+ joint infiltrating cells in Rag2−/− mice transferred with CD4+ T cells from WT ...read more
GM-CSF from ILCs and Radio-Resistant Stromal Cells Is Crucial for Autoimmune Arthritis(A) Preparation of experimental groups for assessing arthritogenic effects of GM-CSF from ILCs or radio-resistant stromal cells. ...read more
GM-CSF-Producing ILCs in Inflamed Joints(A) Flow cytometry analysis of GM-CSF expression by CD4+ T cells and CD4− cells among CD45+ joint infiltrating cells in Rag2−/− mice transferred with CD4+ T cells from WT ...read more
GM-CSF-Producing ILCs in Synovial Fluid of RA Patients(A) The presence of ILCs (defined as CD45+CD3−CD4−CD8−CD11b−CD11c−CD19−CD56−) from peripheral blood (PB) or synovial fluid (SF) of a patient with RA or ...read more
GM-CSF from ILCs and Radio-Resistant Stromal Cells Is Crucial for Autoimmune Arthritis(A) Preparation of experimental groups for assessing arthritogenic effects of GM-CSF from ILCs or radio-resistant stromal cells. ...read more
GM-CSF-Producing ILCs in Inflamed Joints(A) Flow cytometry analysis of GM-CSF expression by CD4+ T cells and CD4− cells among CD45+ joint infiltrating cells in Rag2−/− mice transferred with CD4+ T cells from WT ...read more
GM-CSF-Producing ILCs in Inflamed Joints(A) Flow cytometry analysis of GM-CSF expression by CD4+ T cells and CD4− cells among CD45+ joint infiltrating cells in Rag2−/− mice transferred with CD4+ T cells from WT ...read more
GM-CSF from Non-T Cells Is Crucial for the Initiation of Autoimmune Arthritis(A) Experimental design of adoptive transfer of CD4+ T cells from WT or Csf2−/− SKG mice into Rag2−/− or Csf2−/−Rag2−/− mice. ...read more
GM-CSF-Producing ILCs in Synovial Fluid of RA Patients(A) The presence of ILCs (defined as CD45+CD3−CD4−CD8−CD11b−CD11c−CD19−CD56−) from peripheral blood (PB) or synovial fluid (SF) of a patient with RA or ...read more
GM-CSF-Producing ILCs in Inflamed Joints(A) Flow cytometry analysis of GM-CSF expression by CD4+ T cells and CD4− cells among CD45+ joint infiltrating cells in Rag2−/− mice transferred with CD4+ T cells from WT ...read more
Mouse myeloma cell line NS0-derived recombinant mouse GM-CSF R alpha Leu30-Pro327 Accession # Q00941
Specificity
Detects mouse GM-CSF R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) GM-CSF R alpha or rhGM‑CSF R beta is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
CSF2RA
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Granulocyte macrophage colony stimulating factor receptor alpha (GM-CSF R alpha ), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM-CSF. GM-CSF promotes the differentiation and mobilization of granulocyte-macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effector functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1-4). Mature mouse GM-CSF R alpha is an 80 kDa type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains and a juxtamembrane WSXWS motif, a 21 aa transmembrane segment, and a 40 aa cytoplasmic domain (5). Within the ECD, mouse GM-CSF R alpha shares approximately 33% and 58% aa sequence identity with human and rat GM-CSF R alpha , respectively. Soluble forms of the human receptor retain the ability to bind GM-CSF (6, 7). GM-CSF R alpha is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non‑hematopoietic solid tumor cells (8). GM-CSF R alpha associates with the common beta chain/CD131 ( beta c), a 135 kDa transmembrane protein that is also the signal transducing component of the receptors for IL-3 and IL-5 (9, 10). Association with beta c converts GM-CSF R alpha from a low affinity to a high affinity receptor for GM-CSF (9-11). The shared usage of beta c underlies the synergism between GM-CSF, IL-3, and IL-5 in their effects on myeloid cell differentiation and activation (1, 2).
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Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
Cao, Y. (2007) J. Clin. Invest. 117:2362.
Park, L.S. et al. (1992) Proc. Natl. Acad. Sci. 89:4295.
Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
Raines, M.A. et al. (1991) Proc. Natl. Acad. Sci. 88:8203.
Chiba, S. et al. (1990) Cell Regul. 1:327.
Kitamura, T. et al. (1991) Proc. Natl. Acad. Sci. 88:5082.
Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
Hoang, T. et al. (1993) J. Biol. Chem. 268:11881.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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