![Western Blot: GLDC Antibody [NBP1-32907] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membranes were blotted with Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GLDC-Antibody-Western-Blot-NBP1-32907-img0023.jpg "Western Blot: GLDC Antibody [NBP1-32907] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membranes were blotted with Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, Simple Western, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GLDC. The exact sequence is proprietary. |
| Localization | Mitochondrion |
| Predicted Species | Bovine (92%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GLDC |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. |
| Theoretical MW | 113 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Myoglobin Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Myoglobin Antibody [Unconjugated]")
![Simple Western alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]](https://images.novusbio.com/images2/16253.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for GLDC Antibody (NBP1-32907)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | GLDC |
![Immunocytochemistry/Immunofluorescence: GLDC Antibody [NBP1-32907] - Hep G2 cells were fixed in ice-cold MeOH for 5 min. Green: Glycine dehydrogenase protein stained by Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:400. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/GLDC-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32907-img0020.jpg "Immunocytochemistry/Immunofluorescence: GLDC Antibody [NBP1-32907] - Hep G2 cells were fixed in ice-cold MeOH for 5 min. Green: Glycine dehydrogenase protein stained by Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:400. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907] - Paraffin-embedded mouse brain. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/GLDC-Antibody-Immunohistochemistry-Paraffin-NBP1-32907-img0017.jpg "Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907] - Paraffin-embedded mouse brain. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500.")
![Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907] - Paraffin-embedded mouse liver. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/GLDC-Antibody-Immunohistochemistry-Paraffin-NBP1-32907-img0015.jpg "Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907] - Paraffin-embedded mouse liver. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500.")
![Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907] - Paraffin-embedded rat brain. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500](http://images.novusbio.com/fullsize/GLDC-Antibody-Immunohistochemistry-Paraffin-NBP1-32907-img0016.jpg "Immunohistochemistry-Paraffin: GLDC Antibody [NBP1-32907] - Paraffin-embedded rat brain. Glycine dehydrogenase antibody [N3C2-2], Internal diluted at 1:500")
![Simple Western: GLDC Antibody [NBP1-32907] - Simple Western lane view shows a specific band for GLDC in 0.2 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GLDC-Antibody-Simple-Western-NBP1-32907-img0010.jpg "Simple Western: GLDC Antibody [NBP1-32907] - Simple Western lane view shows a specific band for GLDC in 0.2 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Immunoprecipitation: GLDC Antibody [NBP1-32907] - Immunoprecipitation of Glycine dehydrogenase protein from HepG2 whole cell extracts using 5 ug of Glycine dehydrogenase antibody [N3C2-2], Internal. Western blot analysis was performed using Glycine dehydrogenase antibody [N3C2-2], Internal. EasyBlot anti-Rabbit IgG(was used as a secondary reagent.](http://images.novusbio.com/fullsize/GLDC-Antibody-Immunoprecipitation-NBP1-32907-img0014.jpg "Immunoprecipitation: GLDC Antibody [NBP1-32907] - Immunoprecipitation of Glycine dehydrogenase protein from HepG2 whole cell extracts using 5 ug of Glycine dehydrogenase antibody [N3C2-2], Internal. Western blot analysis was performed using Glycine dehydrogenase antibody [N3C2-2], Internal. EasyBlot anti-Rabbit IgG(was used as a secondary reagent.")
![SDS-PAGE LIGHT/TNFSF14 [Unconjugated]](https://images.novusbio.com/images2/LIGHT_664-LI_719.jpg)
![Bioactivity LIGHT/TNFSF14 [Unconjugated]](https://images.novusbio.com/images2/LIGHT_664-LI_721.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")