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Gelsolin/GSN Antibody (GT1656)

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Genetic Strategies: Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was ...read more
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (GT1656) [NBP3-13559] - Gelsolin/GSN antibody [GT1656] detects Gelsolin/GSN protein at cytoplasm in rat muscle by immunohistochemical analysis. Sample: ...read more
Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin/GSN antibody [GT1656] (NBP3-13559) diluted at 1:1000.
Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin/GSN antibody [GT1656] (NBP3-13559) diluted at 1:1000. ...read more
Western Blot: Gelsolin/GSN Antibody (GT1656) [NBP3-13559] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Gelsolin/GSN antibody [GT1656] (NBP3-13559) diluted at 1:1000. The ...read more
Immunohistochemistry-Paraffin: Gelsolin/GSN Antibody (GT1656) [NBP3-13559] - Gelsolin/GSN antibody [GT1656] detects secreted Gelsolin/GSN protein in rat duodenum by immunohistochemical analysis. Sample: ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ShSpecies Glossary
Applications WB, IHC, KO
Clone
GT1656
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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Gelsolin/GSN Antibody (GT1656) Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Recombinant protein encompassing a sequence within the center region of human Gelsolin/GSN. The exact sequence is proprietary.
Predicted Species
Bovine (95%), Sheep (96%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GSN
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:3000
Theoretical MW
86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for Gelsolin/GSN Antibody (GT1656)

  • Actin-depolymerizing factor
  • ADF
  • AGEL
  • Brevin
  • DKFZp313L0718
  • gelsolin (amyloidosis, Finnish type)
  • Gelsolin
  • GSN

Background

Gelsolin is a calcium regulated actin binding protein. Gelsolin is a regulator of actin filament assembly and disassembly and consists of six homologous subdomains (S1-S6). When C-terminal tail (S6) is bound to calcium ion, it straightens and exposes actin binding sites located on S2s helices, leading to Gelsolin actin-serving activity (1). Gelsolin is inhibited by phosphatidylinositol (4,5)-bisphosphate (PIP2), where PIP2 will bind to S1, S2, and S3 sites. Gelsolin is involved in podosome formation and has been linked to inhibition of apoptosis by preventing release of cytochrome C (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Gelsolin/GSN Antibody (NBP3-13559) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GSN