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GCLM Antibody (CPTC-GCLM-1) - Azide and BSA Free

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Western Blot: GCLM Antibody (CPTC-GCLM-1) - Azide and BSA Free [NBP3-08471] - Western Blot Analysis of A549, A431 & HepG2 cell lysates using GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).
Immunohistochemistry-Paraffin: GCLM Antibody (CPTC-GCLM-1) - Azide and BSA Free [NBP3-08471] - Formalin-fixed, paraffin-embedded human tonsil stained with GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).
Immunohistochemistry-Paraffin: GCLM Antibody (CPTC-GCLM-1) - Azide and BSA Free [NBP3-08471] - Formalin-fixed, paraffin-embedded human tonsil stained with GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
CPTC-GCLM-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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GCLM Antibody (CPTC-GCLM-1) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07298).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant human full-length GCLM protein (Uniprot: P48507)
Localization
Cytoplasm
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GCLM
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for GCLM Antibody (CPTC-GCLM-1) - Azide and BSA Free

  • Gamma-ECS regulatory subunit
  • Gamma-glutamylcysteine synthetase regulatory subunit
  • GCS light chain
  • GLCLRglutamate--cysteine ligase regulatory subunit
  • glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory(30.8kD)
  • Glutamate--cysteine ligase modifier subunit
  • glutamate-cysteine ligase regulatory protein
  • glutamate-cysteine ligase, modifier subunit
  • GSC light chain

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GCLM