![Western Blot: GAPDH Antibody (1A10) - Azide and BSA Free [NBP2-80748] - Analaysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. Theoretical molecular weight: 36 kDa. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Azide-and-BSA-Free-Western-Blot-NBP2-80748-img0001.jpg "Western Blot: GAPDH Antibody (1A10) - Azide and BSA Free [NBP2-80748] - Analaysis using anti-GAPDH mAb against Hela (1), A549 (2), A431 (3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate. Theoretical molecular weight: 36 kDa. Image from the standard format of this antibody.")
| Reactivity | Hu, Mu(-), Rt(-)Species Glossary |
| Applications | WB, Simple Western, ELISA, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | 1A10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | GAPDH antibody developed against purified recombinant fragment of human GAPDH expressed in E. coli. [UniProt# P04406] |
| Localization | Cytoplasmic |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GAPDH |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
| Theoretical MW | 36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for GAPDH Antibody (NBP2-80748)Find related products by research area.
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Considerations for Quantitative Western blotting By Jamshed Arslan, Pharm. D., PhD. Since its inception in 1979, Western blotting has undergone several developments. The use of radioactive probes was common throughout 1980s, but utilizing secondary antibodies labell... Read full blog post. |
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The use of Beta Actin (AC-15) as a loading control across multiple species Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs. Actin has six highly conserved isoforms, however beta and gamma actin are the two... Read full blog post. |
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Tips on choosing an ideal loading control antibody for Western Blotting Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p... Read full blog post. |
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GAPDH - A "Housekeeping" Gene With Diverse Functions in Cellular Homeostasis Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a well-known housekeeping gene with functions in glycolysis. Many biologists are familiar with the gene and use GAPDH antibodies for a loading control when performing western blots. However, this ... Read full blog post. |
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A New Standard in Antibody Testing - Simple Western Certified Antibodies The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi... Read full blog post. |
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GAPDH: More than a housekeeping gene GAPDH is a 146kD tetramer glycolytic pathway metabolic enzyme composed of four 30-40 kDa subunits. It is responsible for reversibly phosphorylating its substrate glyceraldehyde 3-phosphate within the glycolytic pathway. Apart from its role in gl... Read full blog post. |
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GAPDH (Glyceraldehyde 3-Phosphate Dehydrogenase) GAPDH is a 146 kD tetramer glycolytic pathway metabolic enzyme responsible for reversibly phosphorylating glyceraldehyde 3-phosphate. It may have other possible functions in transcriptional activation. GAPDH is highly expressed due to this housekeepin... Read full blog post. |
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Time to Start Actin Like a Reliable 'Housekeeper'! A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properti... Read full blog post. |
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Beta Actin and GAPDH: The Importance of Western Blot Loading Controls What are Western Blot Loading Controls?Western blotting is an essential technique to probe protein expression in complex cell or tissue lysates. To accurately determine protein expression and interpret Western blot results, it is important to use... Read full blog post. |
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The GAPDH Antibody in Western Blot Assays The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | GAPDH |
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1A10) - Azide and BSA Free [NBP2-80748] - GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 2 ug/mL of mouse anti-GAPDH monoclonal (NB300-328), 2 ug/mL mL of mouse anti- GAPDH monoclonal (NBP1-47339). Cells were stained using the appropriate secondary antibody donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80748-img0003.jpg "Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1A10) - Azide and BSA Free [NBP2-80748] - GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 2 ug/mL of mouse anti-GAPDH monoclonal (NB300-328), 2 ug/mL mL of mouse anti- GAPDH monoclonal (NBP1-47339). Cells were stained using the appropriate secondary antibody donkey anti-mouse IgG-NL557 (NL007) and counterstained with DAPI (blue). Image from the standard format of this antibody.")
![Immunohistochemistry-Paraffin: GAPDH Antibody (1A10) - Azide and BSA Free [NBP2-80748] - Analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using anti-GAPDH mAb with DAB staining. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80748-img0004.jpg "Immunohistochemistry-Paraffin: GAPDH Antibody (1A10) - Azide and BSA Free [NBP2-80748] - Analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using anti-GAPDH mAb with DAB staining. Image from the standard format of this antibody.")
![Flow Cytometry: GAPDH Antibody (1A10) - Azide and BSA Free [NBP2-80748] - An intracellular stain was performed on U-87 cells with GAPDH [1A10] Antibody NBP1-47339AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80748-img0005.jpg "Flow Cytometry: GAPDH Antibody (1A10) - Azide and BSA Free [NBP2-80748] - An intracellular stain was performed on U-87 cells with GAPDH [1A10] Antibody NBP1-47339AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1A10) - Azide and BSA Free [NBP2-80748] - Analysis of methanol-fixed HepG2 (left) and HeLa (right) cells using anti-GAPDH mAb (green), showing cytoplasmic localization. DRAQ5 fluorescent DNA dye (blue). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80748-img0002.jpg "Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1A10) - Azide and BSA Free [NBP2-80748] - Analysis of methanol-fixed HepG2 (left) and HeLa (right) cells using anti-GAPDH mAb (green), showing cytoplasmic localization. DRAQ5 fluorescent DNA dye (blue). Image from the standard format of this antibody.")
![Simple Western: GAPDH Antibody (1A10) - Azide and BSA Free [NBP2-80748] - Lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted 36 kDa molecular weight. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1A10-Azide-and-BSA-Free-Simple-Western-NBP2-80748-img0006.jpg "Simple Western: GAPDH Antibody (1A10) - Azide and BSA Free [NBP2-80748] - Lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted 36 kDa molecular weight. Image from the standard format of this antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
![Western Blot: Recombinant Human GAPDH Protein [H00002597-P02] - Analysis of GAPDH in GAPDH recombinant protein. Image from verified customer review.](https://images.novusbio.com/images/Recombinant-Human-GAPDH-Protein-Western-Blot-H00002597-P02-img0003.jpg "Western Blot: Recombinant Human GAPDH Protein [H00002597-P02] - Analysis of GAPDH in GAPDH recombinant protein. Image from verified customer review.")