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Galectin-3 Antibody (194804) [Unconjugated]

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Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Galectin-3 Monoclonal Antibody (Catalog # MAB1154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
194804
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB1154-SP
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Galectin-3 Antibody (194804) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Galectin‑3
Ala2-Ile250
Accession # P17931
Specificity
Detects human Galectin-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows 100% cross-reactivity with recombinant mouse Galectin-3 and no cross-reactivity with rhGalectin-1, -2 -4, -7, or -8.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
LGALS3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
Publications
Read Publications using
MAB1154 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-3 Antibody (194804) [Unconjugated]

  • AGE-R3
  • Carbohydrate-binding protein 35
  • CBP 35,35 kDa lectin
  • CBP35
  • GAL3
  • Gal-3
  • galactin-3
  • Galactose-specific lectin 3
  • Galactoside-binding protein
  • GALBPCBP35
  • Galectin3
  • Galectin-3
  • GALIG
  • IgE-binding protein
  • L29
  • L31
  • L-31
  • Laminin-binding protein
  • Lectin L-29
  • lectin, galactoside-binding, soluble, 3
  • LGALS2
  • LGALS3
  • Mac-2 antigen
  • Mac-2
  • MAC2GAL3

Background

Galectin-3, also known as Mac-2, L29, CBP35 and epsilon BP, is a member of a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells and fibroblasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Galectin-3 Antibody (MAB1154)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, Western Blot.


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Bioinformatics

Gene Symbol LGALS3
Uniprot