![Western Blot: Galectin-1 Antibody [NBP2-16581] - Wild-type (WT) and Galectin-1 knockout (KO) 293T cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin-1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP2-16581-img0014.jpg "Western Blot: Galectin-1 Antibody [NBP2-16581] - Wild-type (WT) and Galectin-1 knockout (KO) 293T cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin-1 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IP, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Full length human Galectin-1 Recombinant protein. |
| Localization | Secreted; extracellular space; extracellular matrix |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | LGALS1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot Galectin-7 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin7_AF1339_Western_Blot_17742.jpg)
![Simple Western Galectin-7 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin7_AF1339_Simple_Western_18701.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Galectin-1 Antibody (NBP2-16581)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody [NBP2-16581] - Galectin 1 antibody detects Galectin-1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Galectin-1 protein stained by Galectin-1 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16581-img0013.jpg "Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody [NBP2-16581] - Galectin 1 antibody detects Galectin-1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Galectin-1 protein stained by Galectin-1 antibody diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.")
![Western Blot: Galectin-1 Antibody [NBP2-16581] - A: BCL-1.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP2-16581-img0006.jpg "Western Blot: Galectin-1 Antibody [NBP2-16581] - A: BCL-1.")
![Western Blot: Galectin-1 Antibody [NBP2-16581] - A: NCI-H929.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP2-16581-img0007.jpg "Western Blot: Galectin-1 Antibody [NBP2-16581] - A: NCI-H929.")
![Western Blot: Galectin-1 Antibody [NBP2-16581] - A. PC-12. B. Rat2.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP2-16581-img0010.jpg "Western Blot: Galectin-1 Antibody [NBP2-16581] - A. PC-12. B. Rat2.")
![Western Blot: Galectin-1 Antibody [NBP2-16581] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin-1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP2-16581-img0012.jpg "Western Blot: Galectin-1 Antibody [NBP2-16581] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin-1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody [NBP2-16581] - A431.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16581-img0005.jpg "Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody [NBP2-16581] - A431.")
![Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody [NBP2-16581] - Galectin 1 antibody detects Galectin 1 protein at cytoplasm. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Galectin 1 protein stained by Galectin 1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16581-img0011.jpg "Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody [NBP2-16581] - Galectin 1 antibody detects Galectin 1 protein at cytoplasm. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Galectin 1 protein stained by Galectin 1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.")
![Immunoprecipitation: Galectin-1 Antibody [NBP2-16581] - Galectin 1 antibody immunoprecipitates Galectin 1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of Galectin 1 antibody expressing HeLa cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of Galectin 1 by 2.5 ug of Galectin 1 antibody](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Immunoprecipitation-NBP2-16581-img0009.jpg "Immunoprecipitation: Galectin-1 Antibody [NBP2-16581] - Galectin 1 antibody immunoprecipitates Galectin 1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of Galectin 1 antibody expressing HeLa cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of Galectin 1 by 2.5 ug of Galectin 1 antibody")
![Western Blot: Galectin-1 Antibody [NBP2-16581] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16581_rabbit-polyclonal-galectin-1-antibody-1610202419414557.jpg "Western Blot: Galectin-1 Antibody [NBP2-16581] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")