![Western Blot: Galectin-1 Antibody [NBP2-16580] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP2-16580-img0015.jpg "Western Blot: Galectin-1 Antibody [NBP2-16580] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Galectin-1. The exact sequence is proprietary. |
| Localization | Secreted; extracellular space; extracellular matrix |
| Predicted Species | Rhesus Macaque (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | LGALS1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot Galectin-7 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin7_AF1339_Western_Blot_17742.jpg)
![Simple Western Galectin-7 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin7_AF1339_Simple_Western_18701.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Galectin-1 Antibody (NBP2-16580)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody [NBP2-16580] - Galectin 1 antibody detects Galectin 1 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Galectin 1 stained by Galectin 1 antibody diluted at 1:1000.Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16580-img0016.jpg "Immunocytochemistry/Immunofluorescence: Galectin-1 Antibody [NBP2-16580] - Galectin 1 antibody detects Galectin 1 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Galectin 1 stained by Galectin 1 antibody diluted at 1:1000.Blue: Fluoroshield with DAPI. Scale bar= 10 um.")
![Immunohistochemistry-Frozen: Galectin-1 Antibody [NBP2-16580] - Frozen sectioned E13.5 Rat brain. Green: Galectin 1 protein stained by Galectin 1 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Immunohistochemistry-Frozen-NBP2-16580-img0010.jpg "Immunohistochemistry-Frozen: Galectin-1 Antibody [NBP2-16580] - Frozen sectioned E13.5 Rat brain. Green: Galectin 1 protein stained by Galectin 1 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Western Blot: Galectin 1 Antibody [NBP2-16580] - Sample (30 ug of whole cell lysate) A: BCL-1 15% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP2-16580-img0001.jpg "Western Blot: Galectin 1 Antibody [NBP2-16580] - Sample (30 ug of whole cell lysate) A: BCL-1 15% SDS PAGE gel, diluted at 1:10000.")
![Western Blot: Galectin 1 Antibody [NBP2-16580] - Sample (30 ug of whole cell lysate) A: NCI-H929 15% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP2-16580-img0002.jpg "Western Blot: Galectin 1 Antibody [NBP2-16580] - Sample (30 ug of whole cell lysate) A: NCI-H929 15% SDS PAGE gel, diluted at 1:10000.")
![Western Blot: Galectin-1 Antibody [NBP2-16580] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP2-16580-img0005.jpg "Western Blot: Galectin-1 Antibody [NBP2-16580] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody diluted at 1:10000.")
![Western Blot: Galectin-1 Antibody [NBP2-16580] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP2-16580-img0012.jpg "Western Blot: Galectin-1 Antibody [NBP2-16580] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Galectin-1 Antibody [NBP2-16580] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin-1 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Western-Blot-NBP2-16580-img0014.jpg "Western Blot: Galectin-1 Antibody [NBP2-16580] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin-1 antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Galectin-1 Antibody [NBP2-16580] - Human colon carcinoma, using Galectin 1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Galectin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-16580-img0003.jpg "Immunohistochemistry-Paraffin: Galectin-1 Antibody [NBP2-16580] - Human colon carcinoma, using Galectin 1antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")