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Galactosylceramidase/GALC Antibody

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Immunohistochemistry-Paraffin: Galactosylceramidase/GALC Antibody [NBP2-16578] - Human ovarian cancer diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Galactosylceramidase/GALC Antibody [NBP2-16578] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ...read more
Galactosylceramidase/GALC antibody detects Galactosylceramidase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.Galactosylceramidase stained by Galactosylceramidase antibody ...read more
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Galactosylceramidase/GALC antibody diluted at 1:3000. An HRP-conjugated anti-rabbit IgG antibody was used to detect ...read more

Product Details

Summary
Reactivity Hu, Mu, RMSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Galactosylceramidase/GALC Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Galactosylceramidase/GALC. The exact sequence is proprietary.
Localization
Lysosome
Predicted Species
Rhesus Macaque (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GALC
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500 - 1:3000
Theoretical MW
77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Canine (87%), Bovine (84%), Pig (87%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for Galactosylceramidase/GALC Antibody

  • EC 3.2.1.46
  • Galactocerebrosidase
  • Galactocerebroside beta-galactosidase
  • galactosylceramidase (Krabbe disease)
  • Galactosylceramidase
  • Galactosylceramide beta-galactosidase
  • Galactosylceraminidase
  • GALC
  • GALCERase

Background

This gene encodes a lysosomal protein which hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Mutations in this gene have been associated with Krabbe disease, also known as globoid cell leukodystrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GALC
Uniprot