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FOXO3 Antibody [Unconjugated]

Name: FOXO3 Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF6165
Price: 479 USD

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Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, and PC-3 human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human FoxO3 ...read more

FoxO3 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human FoxO3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6165) at 10 µg/mL for 3 hours at room temperature. Cells ...read more

Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for FoxO3 at approximately 97 kDa (as indicated) using 10 µg/mL of Sheep ...read more

Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and FoxO3 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse ...read more

Product Details

Summary

Reactivity	Hu, MuSpecies Glossary
Applications	WB, Simple Western, ICC/IF, KO
Clonality	Polyclonal
Host	Sheep
Conjugate	Unconjugated
Concentration	LYOPH

Validated by:

Genetic Strategies

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Catalog# & Formulation	Size	Price
Carrier Free AF6165-SP

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FOXO3 Antibody [Unconjugated] Summary

Immunogen	E. coli-derived recombinant human FoxO3 Met372-Gly673 Accession # O43524
Specificity	Detects human and mouse FoxO3 in Western blots.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Sheep
Gene	FOXO3
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Immunocytochemistry 5-15 ug/mL
Knockout Validated
Simple Western 10 ug/mL
Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FOXO3 Antibody [Unconjugated]

AF6q21 protein
AF6q21
DKFZp781A0677
FKHRL1
FKHRL1MGC31925
FKHRL1P2
forkhead box O3
forkhead box protein O3
forkhead homolog (rhabdomyosarcoma) like 1
Forkhead in rhabdomyosarcoma-like 1
forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
FOXO2
FoxO3
FOXO3A
FOXO3Aforkhead box O3A
MGC12739

Background

Forkhead box O3 (FoxO3) is a ubiquitously expressed 72 kDa transcriptional regulator that is involved in cellular differentiation, angiogenesis, tumor progression, apoptosis, and the responses to oxidative stress and DNA damage. Phosphorylation of FoxO3 by Akt induces its association with 14-3-3 proteins and its retention in the cytoplasm. In response to the loss of survival factors, dephosphorylation of FoxO3 induces its translocation to the nucleus where it promotes apoptosis. Its level of acetylation is regulated in response to cellular metabolic requirements. Within amino acids 372‑673 (C-terminal to the DNA binding domain), human FoxO3 shares 95% aa sequence identity with mouse and rat FoxO3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

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Sheep IgG Isotype Control [Unconjugated]
5-001-A

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Bioinformatics

Gene Symbol	FOXO3
Uniprot	O43524(Human)

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

FOXO3 Antibody [Unconjugated]

Species Glossary

Images

Product Details

Order Details

FOXO3 Antibody [Unconjugated] Summary

Applications/Dilutions

Packaging, Storage & Formulations

Notes

Alternate Names for FOXO3 Antibody [Unconjugated]

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for FOXO3 Antibody (AF6165) (0)

Reviews for FOXO3 Antibody (AF6165) (0)

Product General Protocols

Video Protocols

FAQs for FOXO3 Antibody (AF6165) (0)

Secondary Antibodies

Isotype Controls

Additional FOXO3 Products

Blogs on FOXO3

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics