![Western Blot: FOXO1A/3/4 Antibody [NBP3-05667] - Western blot analysis of extracts of various cell lines, using FOXO1A/3/4 antibody (NBP3-05667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/FOXO1A-3-4-Antibody-Western-Blot-NBP3-05667-img0004.jpg "Western Blot: FOXO1A/3/4 Antibody [NBP3-05667] - Western blot analysis of extracts of various cell lines, using FOXO1A/3/4 antibody (NBP3-05667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | A synthetic peptide of human FOXO1A/3/4 (NP_002006.2). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FOXO1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Affinity purified |
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
![Immunohistochemistry-Paraffin FOXO4 [p Ser197] Antibody - Azide and BSA Free](https://images.novusbio.com/images/FOXO4-[p-Ser197]-Antibody-Immunohistochemistry-Paraffin-NBP3-05393-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence FOXO4 [p Ser197] Antibody - Azide and BSA Free](https://images.novusbio.com/images/FOXO4-[p-Ser197]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05393-img0002.jpg)
![Western Blot FOXO4 [p Ser197] Antibody - Azide and BSA Free](https://images.novusbio.com/images/FOXO4-[p-Ser197]-Antibody-Western-Blot-NBP3-05393-img0003.jpg)
![Western Blot PTEN Antibody [Unconjugated]](https://images.novusbio.com/images2/PTEN_AF847_Western_Blot_5922.jpg)
![Simple Western PTEN Antibody [Unconjugated]](https://images.novusbio.com/images2/16342.jpg)
![Knockout Validated PTEN Antibody [Unconjugated]](https://images.novusbio.com/images2/PTEN_AF847_Knockout_Validated_22996.jpg)
| Publication using NBP3-05667 | Applications | Species |
|---|---|---|
| Imrie H, Viswambharan H, Haywood NJ et al. Cixutumumab reveals a critical role for IGF-1 in adipose and hepatic tissue remodelling during the development of diet-induced obesity Adipocyte 2022-12-01 [PMID: 35734881] (WB, Mouse) | WB | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for FOXO1A/3/4 Antibody (NBP3-05667)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | FOXO1 |
![Immunohistochemistry-Paraffin: FOXO1A/3/4 Antibody [NBP3-05667] - Immunohistochemistry of paraffin-embedded mouse spinal cord using FOXO1A/3/4 Rabbit pAb (NBP3-05667) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/FOXO1A-3-4-Antibody-Immunohistochemistry-Paraffin-NBP3-05667-img0003.jpg "Immunohistochemistry-Paraffin: FOXO1A/3/4 Antibody [NBP3-05667] - Immunohistochemistry of paraffin-embedded mouse spinal cord using FOXO1A/3/4 Rabbit pAb (NBP3-05667) at dilution of 1:100 (40x lens).")
![Immunohistochemistry-Paraffin: FOXO1A/3/4 Antibody [NBP3-05667] - Immunohistochemistry of paraffin-embedded rat brain using FOXO1A/3/4 Rabbit pAb (NBP3-05667) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/FOXO1A-3-4-Antibody-Immunohistochemistry-Paraffin-NBP3-05667-img0001.jpg "Immunohistochemistry-Paraffin: FOXO1A/3/4 Antibody [NBP3-05667] - Immunohistochemistry of paraffin-embedded rat brain using FOXO1A/3/4 Rabbit pAb (NBP3-05667) at dilution of 1:100 (40x lens).")
![Immunohistochemistry-Paraffin: FOXO1A/3/4 Antibody [NBP3-05667] - Immunohistochemistry of paraffin-embedded human colon carcinoma using FOXO1A/3/4 Rabbit pAb (NBP3-05667) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/FOXO1A-3-4-Antibody-Immunohistochemistry-Paraffin-NBP3-05667-img0002.jpg "Immunohistochemistry-Paraffin: FOXO1A/3/4 Antibody [NBP3-05667] - Immunohistochemistry of paraffin-embedded human colon carcinoma using FOXO1A/3/4 Rabbit pAb (NBP3-05667) at dilution of 1:100 (40x lens).")
![Immunohistochemistry: FOXO1A/3/4 Antibody - Azide and BSA Free [FOXO1A/3/4] - Immunohistochemistry analysis of paraffin-embedded human placenta using FOXO1A/3/4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-05667_rabbit-polyclonal-foxo1a-3-4-antibody-52202516293911.jpg "Immunohistochemistry: FOXO1A/3/4 Antibody - Azide and BSA Free [FOXO1A/3/4] - Immunohistochemistry analysis of paraffin-embedded human placenta using FOXO1A/3/4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![Immunohistochemistry: FOXO1A/3/4 Antibody - Azide and BSA Free [FOXO1A/3/4] - Immunohistochemistry analysis of paraffin-embedded mouse liver using FOXO1A/3/4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-05667_rabbit-polyclonal-foxo1a-3-4-antibody-5220251628030.jpg "Immunohistochemistry: FOXO1A/3/4 Antibody - Azide and BSA Free [FOXO1A/3/4] - Immunohistochemistry analysis of paraffin-embedded mouse liver using FOXO1A/3/4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![Immunohistochemistry: FOXO1A/3/4 Antibody - Azide and BSA Free [FOXO1A/3/4] - Immunohistochemistry analysis of paraffin-embedded rat testis using FOXO1A/3/4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-05667_rabbit-polyclonal-foxo1a-3-4-antibody-52202516293910.jpg "Immunohistochemistry: FOXO1A/3/4 Antibody - Azide and BSA Free [FOXO1A/3/4] - Immunohistochemistry analysis of paraffin-embedded rat testis using FOXO1A/3/4 Rabbit pAb at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.")
![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
![Immunohistochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/mab1417_human-bovine-mouse-insulin-mab-clone-182410-immunohistochemistry-308202115145.jpg)
![Simple Western PYK2/FAK2 [p Tyr402] Antibody (592918) [Unconjugated]](https://images.novusbio.com/images2/PYK2_MAB6210_Simple_Western_16639.jpg)
![Western Blot PYK2/FAK2 [p Tyr402] Antibody (592918) [Unconjugated]](https://images.novusbio.com/images2/PYK2_MAB6210_Western_Blot_9430.jpg)
![Immunocytochemistry Pax3/Pax7 Antibody (274212) [Unconjugated]](https://images.novusbio.com/images2/Pax3_MAB2457_Immunocytochemistry__Immunofluorescence_20313.jpg)
![Immunocytochemistry/ Immunofluorescence Pax3/Pax7 Antibody (274212) [Unconjugated]](https://images.novusbio.com/images/mab2457_human-mouse-pax3-pax7-mab-clone-274212-41202412512639.jpg)
![SDS-PAGE IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_40.jpg)
![Bioactivity IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_41.jpg)
![Mass Spectrometry IGF-I/IGF-1 [Unconjugated]](https://images.novusbio.com/images2/IGF-I_291-G1_42.jpg)
![Immunohistochemistry-Paraffin TOR/mTOR [p Ser2448] Antibody](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-Paraffin-NB600-607-img0005.jpg)
![Western Blot TOR/mTOR [p Ser2448] Antibody](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0006.jpg)
![Data TOR/mTOR [p Ser2448] Antibody](https://images.novusbio.com/images/TOR-mTOR-[p-Ser2448]-Antibody-N-A-NB600-607-img0008.jpg)
![Immunocytochemistry Pax7 Antibody (PAX7) [Unconjugated]](https://images.novusbio.com/images2/Pax7_MAB1675_Immunocytochemistry_6571.jpg)
![Immunocytochemistry Pax7 Antibody (PAX7) [Unconjugated]](https://images.novusbio.com/images2/Pax7_MAB1675_Immunocytochemistry__Immunofluorescence_20311.jpg)
![Immunohistochemistry Pax7 Antibody (PAX7) [Unconjugated]](https://images.novusbio.com/images2/mab1675_human-mouse-rat-chicken-pax7-mab-clone-pax7-immunohistochemistry-3082021134114.jpg)
![Western Blot p27/Kip1 [p Thr157] Antibody](https://images.novusbio.com/images2/p27_AF1555_Western_Blot_5119.jpg)
![N/A IGFBP-1 [Biotin]](https://images.novusbio.com/images2/DATA_IGFBP1_DY871_ELISA_2250.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")