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FoxO1/FKHR Antibody

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Western Blot: FoxO1/FKHR Antibody [NB100-81924] - Analysis of extracts from Hela cells using NB100-81924
Western Blot: FoxO1/FKHR Antibody [NB100-81924] - Analysis of extracts from Hela cells using NB100-81924
Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody [NB100-81924] - Staining of methanol-fixed Hela cells using NB100-81924
Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody [NB100-81924] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-81924 (left) or the same antibody preincubated with blocking ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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FoxO1/FKHR Antibody Summary

Immunogen
Peptide sequence around amino acids 254~258 (A-A-S-M-D) derived from Human FoxO1/FKHR
Specificity
Detects endogenous level of total FoxO1/FKHR protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXO1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
FoxO1/FKHR Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FoxO1/FKHR Antibody

  • FKH1
  • FKHR
  • FKHRforkhead box protein O1
  • forkhead box O1
  • Forkhead box protein O1A
  • forkhead homolog in rhabdomyosarcoma
  • Forkhead in rhabdomyosarcoma
  • FoxO1
  • FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Background

FOXO1A (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein which is a member of a subfamily of the forkhead homeotic gene family of transcription factors. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Chromosomal aberrations involving FOXO1A are a cause of rhabdomyosarcoma 2 (RMS2); also known as alveolar rhabdomyosarcoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

FoxO1/FKHR Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNA-binding domain known as the Forkhead Box, or FOX. The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,...  Read full blog post.
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Bioinformatics

Gene Symbol FOXO1
Entrez
Uniprot