![Western Blot: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of FOXO1A on different lysates with Rabbit anti-FOXO1A antibody at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate ysates/proteins at 10 ug/Lane. Predicted band size: 70 kDa observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Western-Blot-NBP2-67463-img0008.jpg "Western Blot: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of FOXO1A on different lysates with Rabbit anti-FOXO1A antibody at 1/500 dilution. Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate ysates/proteins at 10 ug/Lane. Predicted band size: 70 kDa observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, Rt, ZeSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | SU33-01 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human FoxO1/FKHR aa 301-350 / 655. (SwissProt: Q12778 Human; SwissProt: Q9R1E0 Mouse; SwissProt: G3V7R4 Rat) |
| Localization | Cytoplasm, Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | FOXO1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-Fr usage was reported in scientific literature (PMID: 31196960). |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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| Publication using NBP2-67463 | Applications | Species |
|---|---|---|
| Miyauchi T, Uchida Y, Kadono K et al. Up-regulation of FOXO1 and reduced inflammation by beta-hydroxybutyric acid are essential diet restriction benefits against liver injury Proc. Natl. Acad. Sci. U.S.A. 2019-07-02 [PMID: 31196960] (IHC-Fr, Mouse) | IHC-Fr | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for FoxO1/FKHR Antibody (NBP2-67463)Find related products by research area.
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FOXO1/FKHR (fork head in Rhabdomyosarcoma) FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNA-binding domain known as the Forkhead Box, or FOX. The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | FOXO1 |
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunocytochemistry-Immunofluorescence-NBP2-67463-img0003.jpg "Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded mouse heart tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0007.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded mouse heart tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.")
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunocytochemistry-Immunofluorescence-NBP2-67463-img0001.jpg "Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunocytochemistry-Immunofluorescence-NBP2-67463-img0002.jpg "Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Staining FOXO1A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0004.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded human tonsil tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0005.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded human tonsil tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded mouse brain tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-SU33-01-Immunohistochemistry-Paraffin-NBP2-67463-img0006.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (SU33-01) [NBP2-67463] - Analysis of paraffin-embedded mouse brain tissue using anti-FOXO1A antibody. Counter stained with hematoxylin.")
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