![Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Staining in human breast cell lines from nuclear fractions.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-Western-Blot-NBP2-22190-img0006.jpg "Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Staining in human breast cell lines from nuclear fractions.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | 3B6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Purified recombinant fragment of human FoxO1/FKHR expressed in E. Coli. |
| Localization | Cytoplasm |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FOXO1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB reported from a verified customer review. |
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| Theoretical MW | 69.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||
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Enlarge |
reviewed by:
Verified Customer |
WB | Human | 08/23/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for FoxO1/FKHR Antibody (NBP2-22190)Find related products by research area.
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FOXO1/FKHR (fork head in Rhabdomyosarcoma) FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNA-binding domain known as the Forkhead Box, or FOX. The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | FOXO1 |
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Immunofluorescence analysis of Hela cells using FOXO1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-Immunocytochemistry-Immunofluorescence-NBP2-22190-img0001.jpg "Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Immunofluorescence analysis of Hela cells using FOXO1A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.")
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Immunohistochemical analysis of paraffin-embedded intima cancer tissues using FOXO1A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-Immunohistochemistry-Paraffin-NBP2-22190-img0002.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Immunohistochemical analysis of paraffin-embedded intima cancer tissues using FOXO1A mouse mAb with DAB staining.")
![Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Western blot analysis using FOXO1A mAb against human FOXO1 (AA: 471-600) recombinant protein. (Expected MW is 39.3 kDa)](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-Western-Blot-NBP2-22190-img0004.jpg "Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Western blot analysis using FOXO1A mAb against human FOXO1 (AA: 471-600) recombinant protein. (Expected MW is 39.3 kDa)")
![Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Western blot analysis using FOXO1A mouse mAb against Hela (1), HEK293 (2), MCF-7(3), and C6 (4) cell lysate.](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-Western-Blot-NBP2-22190-img0005.jpg "Western Blot: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Western blot analysis using FOXO1A mouse mAb against Hela (1), HEK293 (2), MCF-7(3), and C6 (4) cell lysate.")
![ELISA: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/FoxO1-FKHR-Antibody-3B6-ELISA-NBP2-22190-img0003.jpg "ELISA: FoxO1/FKHR Antibody (3B6) [NBP2-22190] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
