Home » Flt-3/Flk-2/CD135 » Flt-3/Flk-2/CD135 Antibodies » Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [Janelia Fluor® 646]
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Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clone
7E8.2C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-42210AF350
Alexa Fluor 405
NBP2-42210AF405
Alexa Fluor 488
NBP2-42210AF488
Alexa Fluor 532
NBP2-42210AF532
Alexa Fluor 594
NBP2-42210AF594
Alexa Fluor 647
NBP2-42210AF647
Alexa Fluor 700
NBP2-42210AF700
Alexa Fluor 750
NBP2-42210AF750
Allophycocyanin
NBP2-42210APC
Biotin
NBP2-42210B
CoraFluor 1
NBP2-42210CL1
DyLight 350
NBP2-42210UV
DyLight 405
NBP2-42210V
DyLight 488
NBP2-42210G
DyLight 550
NBP2-42210R
DyLight 594
NBP2-42210DL594
DyLight 650
NBP2-42210C
DyLight 680
NBP2-42210FR
DyLight 755
NBP2-42210IR
FITC
NBP2-42210F
HRP
NBP2-42210H
Janelia Fluor 525
NBP2-42210JF525
Janelia Fluor 549
NBP2-42210JF549
Janelia Fluor 669
NBP2-42210JF669
PE
NBP2-42210PE
PerCP
NBP2-42210PCP
Unconjugated
NBP2-42210
mFluor Violet 450 SE
NBP2-42210MFV450
mFluor Violet 500 SE
NBP2-42210MFV500
mFluor Violet 610 SE
NBP2-42210MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-42210
BSA Free
NBP2-42210SS
BSA Free
NBP2-42210
Azide and BSA Free
NBP2-80739
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Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [Janelia Fluor® 646] Summary

Immunogen
Partial recombinant human FLT3 protein (between amino acids 250-500) [UniProt P36888]
Localization
Endoplasmic reticulum lumen, Membrane
Isotype
IgG2b Lambda
Clonality
Monoclonal
Host
Mouse
Gene
FLT3
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: ~86% to Mouse and Rat's FLT3 protein.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Flt-3/Flk-2/CD135 Antibody (7E8.2C8) [Janelia Fluor® 646]

  • CD135 antigen
  • CD135
  • EC 2.7.10
  • fetal liver kinase 2
  • FL cytokine receptor
  • FLK2
  • Flk-2
  • FLT3 receptor tyrosine kinase
  • Flt3
  • Flt-3
  • Fms-like tyrosine kinase 3
  • fms-related tyrosine kinase 3
  • growth factor receptor tyrosine kinase type III
  • Stem cell tyrosine kinase 1
  • STK-1
  • STK1EC 2.7.10.1
  • Tyrosine-protein kinase receptor FLT3

Background

Stem cell tyrosine kinase (STK-1) has been cloned from a CD34+ hematopoietic stem cell enriched library and identified as the human homolog of a previously identified gene of mouse origin designated either Flk-2 or Flt-3. The STK-1 cDNA encodes a protein of 993 amino acids with 85% identity to Flt-3/Flk-2. STK-1 is a member of the type III receptor tyrosine kinase family that includes Kit (steel factor receptor), Fms and PDGF. STK-1 expression in blood and marrow is restricted to CD34+ cells, a population greatly enriched for hematopoietic stem/progenitor cells. STK-1 antiserum recognizes two polypeptides in these cells. The mouse homolog of STK-1, designated Flt-3/ Flk-2, is expressed at high levels in hematopoietic cells and also in neural, gonadal, hepatic and placental tissues. It has been suggested that STK-1 and its murine homolog Flt-3/ Flk-2 may function as growth factor receptors on hematopoietic stem and/or progenitor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FLT3