![Western Blot: Flotillin 2 Antibody [NBP1-30881] - 293T whole cell extracts and 293T exosome extract (2.6 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 2 antibody [C3], C-term (NBP1-30881). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Western-Blot-NBP1-30881-img0013.jpg "Western Blot: Flotillin 2 Antibody [NBP1-30881] - 293T whole cell extracts and 293T exosome extract (2.6 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 2 antibody [C3], C-term (NBP1-30881). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Flotillin 2. The exact sequence is proprietary. |
| Localization | Cell membrane, Peripheral membrane protein, Membrane, caveola, Peripheral membrane protein |
| Predicted Species | Bovine (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FLOT2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
![Simple Western CD44 Antibody (2C5) [Unconjugated] - s Pan Specific](https://images.novusbio.com/images2/CD44_BBA10_Simple_Western_21377.jpg)
![Western Blot CD44 Antibody (2C5) [Unconjugated] - s Pan Specific](https://images.novusbio.com/images2/CD44_BBA10_Western_Blot_21459.jpg)
![Immunohistochemistry CD44 Antibody (2C5) [Unconjugated] - s Pan Specific](https://images.novusbio.com/images2/CD44_BBA10_Immunohistochemistry_17361.jpg)
| Publication using NBP1-30881 | Applications | Species |
|---|---|---|
| Sung SE, Seo MS, Kang KK et al. Isolation and Characterization of Extracellular Vesicle from Mesenchymal Stem Cells of the Epidural Fat of the Spine Asian Spine Journal 2022-04-30 [PMID: 34461688] (B/N, WB) | B/N, WB |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Verified Customer |
WB | Human | 10/11/2019 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: Flotillin 2 Antibody [NBP1-30881] - Analysis of paraformaldehyde-fixed HeLaS3, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30881-img0004.jpg "Immunocytochemistry/Immunofluorescence: Flotillin 2 Antibody [NBP1-30881] - Analysis of paraformaldehyde-fixed HeLaS3, using antibody at 1:100 dilution.")
![Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-30881] - Human lung cancer, using Flotillin-2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-30881-img0006.jpg "Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-30881] - Human lung cancer, using Flotillin-2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: Flotillin 2 Antibody [NBP1-30881] - 50 ug mouse brain lysate/extract. Antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Western-Blot-NBP1-30881-img0007.jpg "Western Blot: Flotillin 2 Antibody [NBP1-30881] - 50 ug mouse brain lysate/extract. Antibody dilution 1:1000.")
![Western Blot: Flotillin 2 Antibody [NBP1-30881] - 50 ug rat brain lysate/extract. Antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Western-Blot-NBP1-30881-img0008.jpg "Western Blot: Flotillin 2 Antibody [NBP1-30881] - 50 ug rat brain lysate/extract. Antibody dilution 1:1000.")
![Western Blot: Flotillin 2 Antibody [NBP1-30881] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and blotted with Flotillin 2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Western-Blot-NBP1-30881-img0012.jpg "Western Blot: Flotillin 2 Antibody [NBP1-30881] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and blotted with Flotillin 2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunoprecipitation: Flotillin 2 Antibody [NBP1-30881] - Immunoprecipitation of Flotillin 2 protein from HeLa whole cell extracts using 5 ug of Flotillin 2 antibody [C3], C-term. Western blot analysis was performed using Flotillin 2 antibody [C3], C-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Immunoprecipitation-NBP1-30881-img0010.jpg "Immunoprecipitation: Flotillin 2 Antibody [NBP1-30881] - Immunoprecipitation of Flotillin 2 protein from HeLa whole cell extracts using 5 ug of Flotillin 2 antibody [C3], C-term. Western blot analysis was performed using Flotillin 2 antibody [C3], C-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![N/A Erythropoietin/EPO [HRP]](https://images.novusbio.com/images2/DATA_Erythropoietin_MEP00_ELISA_970.jpg)
![N/A Erythropoietin/EPO [HRP]](https://images.novusbio.com/images2/Erythropoietin_MEP00_ELISA_421.jpg)
![N/A TIMP-1 [HRP]](https://images.novusbio.com/images2/DATA_TIMP1_DTM100_ELISA_847.jpg)
![N/A TIMP-1 [HRP]](https://images.novusbio.com/images2/TIMP-1_DTM100_ELISA_194.jpg)
![Holo-Transferrin [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Holo-Transferrin [Unconjugated]")
![N/A C-Reactive Protein/CRP [Biotin]](https://images.novusbio.com/images2/DATA_CReactive_Protein_DY1707_ELISA_1701.jpg)
![N/A Hepcidin Antimicrobial Peptide [HRP]](https://images.novusbio.com/images2/Hepcidin_DHP250_ELISA_1121.jpg)
![N/A Hepcidin Antimicrobial Peptide [HRP]](https://images.novusbio.com/images2/Hepcidin_DHP250_ELISA_1122.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
