Flotillin 2 Antibodies
We offer Flotillin 2 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Western Blot. Each Flotillin 2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Flotillin 2 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the Flotillin 2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Flotillin 2 polyclonal antibodies and browse our Flotillin 2 monoclonal antibody catalog.
Alternate Names for Flotillin 2 Antibodies
anti-Flotillin 2 antibody, anti-FLOT2 antibody, anti-Epidermal surface antigen antibody, anti-ESA1Membrane component chromosome 17 surface marker 1 antibody, anti-ESAchromosome 17, surface marker 1 (35kD protein identified bymonoclonal ECS-1) antibody
10 Results for "flotillin-2" in Primary Antibodies
![Western Blot: Flotillin 2 Antibody [NBP1-30881] - 293T whole cell extracts and 293T exosome extract (2.6 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 2 antibody [C3], C-term (NBP1-30881). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Western-Blot-NBP1-30881-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Flotillin 2 Antibody [NBP1-30881] - 293T whole cell extracts and 293T exosome extract (2.6 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Flotillin 2 antibody [C3], C-term (NBP1-30881). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Flotillin 2 Antibody [NBP1-30881] - Analysis of paraformaldehyde-fixed HeLaS3, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30881-img0004.jpg)
![Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-30881] - Human lung cancer, using Flotillin-2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-30881-img0006.jpg)
![Western Blot: Flotillin 2 Antibody [NBP1-30881] - 50 ug rat brain lysate/extract. Antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Western-Blot-NBP1-30881-img0008.jpg)
![Western Blot: Flotillin 2 Antibody [NBP1-30881] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and blotted with Flotillin 2 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Western-Blot-NBP1-30881-img0012.jpg)
![Immunoprecipitation: Flotillin 2 Antibody [NBP1-30881] - Immunoprecipitation of Flotillin 2 protein from HeLa whole cell extracts using 5 ug of Flotillin 2 antibody [C3], C-term. Western blot analysis was performed using Flotillin 2 antibody [C3], C-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Immunoprecipitation-NBP1-30881-img0010.jpg)
![Western Blot: Flotillin 2 Antibody [NBP1-30881] -Flotillin 2 antibody [C3], C-term detects Flotillin 2 protein by Western blot analysis. Mouse tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and blotted with Flotillin 2 antibody [C3], C-term diluted by 1:1000.](http://images.novusbio.com/fullsize/antibody/nbp1-30881_rabbit-polyclonal-flotillin-2-antibody-western-blot-25102024115346..png)
![Immunohistochemistry: Flotillin 2 Antibody (PSH03-41) [NBP3-32343] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Flotillin 2 antibody (NBP3-32343) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32343) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32343_rabbit-flotillin-2-mab-psh03-41-29820241433365.jpg "Immunohistochemistry: Flotillin 2 Antibody (PSH03-41) [NBP3-32343] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Flotillin 2 antibody (NBP3-32343) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32343) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: Flotillin 2 Antibody (PSH03-41) [NBP3-32343] - Immunocytochemistry analysis of HeLa cells labeling Flotillin 2 with Rabbit anti-Flotillin 2 antibody (NBP3-32343) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Flotillin 2 antibody (NBP3-32343) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32343_rabbit-flotillin-2-mab-psh03-41-29202410334063.jpg)
![Flow Cytometry: Flotillin 2 Antibody (PSH03-41) [NBP3-32343] - Flow cytometric analysis of HeLa cells labeling Flotillin 2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32343, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32343_rabbit-flotillin-2-mab-psh03-41-392024884634.jpg)
![Western Blot: Flotillin 2 Antibody (PSH03-41) [NBP3-32343] - Western blot analysis of Flotillin 2 on different lysates with Rabbit anti-Flotillin 2 antibody (NBP3-32343) at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: A431 cell lysate (15 µg/Lane) Lane 3: COS-1 cell lysate (15 µg/Lane) Lane 4: NIH/3T3 cell lysate (15 µg/Lane) Lane 5: PC-12 cell lysate (15 µg/Lane) Lane 6: Mouse lung tissue lysate (30 µg/Lane) Lane 7: Rat lung tissue lysate (30 µg/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: Lane 1-7 (left): 6 seconds; Lane 1-7 (right): 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32343_rabbit-flotillin-2-mab-psh03-41-3920248485016.jpg)
![Western Blot: Flotillin 2 Antibody [NBP2-16507] - Flotillin 2 antibody immunoprecipitates Flotillin 2 protein in IP experiments.IP samples: HCT-116 whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of Flotillin 2 protein by 4 ug Flotillin 2 antibody. 10 % SDS-PAGE. The immunoprecipitated Flotillin 2 protein was detected by Flotillin 2 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Western-Blot-NBP2-16507-img0004.jpg "Western Blot: Flotillin 2 Antibody [NBP2-16507] - Flotillin 2 antibody immunoprecipitates Flotillin 2 protein in IP experiments.IP samples: HCT-116 whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of Flotillin 2 protein by 4 ug Flotillin 2 antibody. 10 % SDS-PAGE. The immunoprecipitated Flotillin 2 protein was detected by Flotillin 2 antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP2-16507] - Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16507-img0003.jpg)
![Western Blot: Flotillin 2 Antibody [NBP2-16507] - Sample (30 ug of whole cell lysate) A: HCT116 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Western-Blot-NBP2-16507-img0001.jpg)
![Western Blot: Flotillin 2 Antibody [NBP2-16507] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Western-Blot-NBP2-16507-img0002.jpg)
![Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-84910] - Staining in human spleen and skeletal muscle tissues using anti-FLOT2 antibody. Corresponding FLOT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-84910-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-84910] - Staining in human spleen and skeletal muscle tissues using anti-FLOT2 antibody. Corresponding FLOT2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Flotillin 2 Antibody [NBP1-84910] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Western-Blot-NBP1-84910-img0006.jpg)
![Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-84910] - Staining of human spleen shows high expression.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-84910-img0008.jpg)
![Western Blot: Flotillin 2 Antibody [NBP1-84910] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Western-Blot-NBP1-84910-img0005.jpg)
![Immunohistochemistry-Paraffin: Flotillin 2 Antibody [NBP1-84910] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-84910-img0007.jpg)
![Western Blot: Flotillin 2 Antibody (3G6) [H00002319-M03] - Analysis of FLOT2 expression in transfected 293T cell line by FLOT2 monoclonal antibody (M03), clone 3G6. Lane 1: FLOT2 transfected lysate (Predicted MW: 41.7 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-3G6-Western-Blot-H00002319-M03-img0004.jpg "Western Blot: Flotillin 2 Antibody (3G6) [H00002319-M03] - Analysis of FLOT2 expression in transfected 293T cell line by FLOT2 monoclonal antibody (M03), clone 3G6. Lane 1: FLOT2 transfected lysate (Predicted MW: 41.7 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: Flotillin 2 Antibody (3G6) [H00002319-M03] - Analysis of FLOT2 transfected lysate using anti-FLOT2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FLOT2 monoclonal antibody.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-3G6-Western-Blot-H00002319-M03-img0001.jpg)
![Western Blot: Flotillin 2 Antibody (3G6) [H00002319-M03] - Analysis of FLOT2 expression in A-431.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-3G6-Western-Blot-H00002319-M03-img0002.jpg)
![Western Blot: Flotillin 2 Antibody (3G6) [H00002319-M03] - Analysis of FLOT2 expression in human stomach.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-3G6-Western-Blot-H00002319-M03-img0003.jpg)
![Western Blot: Flotillin 2 Antibody [NBP3-17714] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Western-Blot-NBP3-17714-img0002.jpg "Western Blot: Flotillin 2 Antibody [NBP3-17714] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG")
![Immunocytochemistry/Immunofluorescence: Flotillin 2 Antibody [NBP3-17714] - Staining of human cell line RT4 shows localization to plasma membrane.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17714-img0001.jpg)
![Western Blot: Flotillin 2 Antibody [NBP2-98723] - Anti-Flotillin 2 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 50 kDa](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Western-Blot-NBP2-98723-img0002.jpg "Western Blot: Flotillin 2 Antibody [NBP2-98723] - Anti-Flotillin 2 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 50 kDa")
![Immunocytochemistry/Immunofluorescence: Flotillin 2 Antibody [NBP2-98723] - Immunofluorescence staining of Flotillin 2 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human Flotillin 2 polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98723-img0001.jpg)
![Western Blot: Flotillin 2 Antibody [NB300-922] - Analysis of Flotillin 2 in human brain (Cerebellum) lysate (35 ug protein in RIPA buffer) using NB300-922 at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Flotillin-2-Antibody-Western-Blot-NB300-922-img0004.jpg "Western Blot: Flotillin 2 Antibody [NB300-922] - Analysis of Flotillin 2 in human brain (Cerebellum) lysate (35 ug protein in RIPA buffer) using NB300-922 at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Flotillin 2 Antibody (SR1814) [NBP3-21613] - Western blot analysis using NBP3-21613 in (1) Hela cell lysate;(2) 293 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21613_rabbit-flotillin-2-mab-sr1814-57202315584827.jpg "Western Blot: Flotillin 2 Antibody (SR1814) [NBP3-21613] - Western blot analysis using NBP3-21613 in (1) Hela cell lysate;(2) 293 cell lysate.")