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FLIP Antibody (7A3D12) - BSA Free

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Western Blot: FLIP Antibody (7A3D12) [NBP2-37359] - Western blot analysis using CFLAR mouse mAb against JURKAT (1), 3T3L1 (2) and RAJI (3) cell lysate.
Western Blot: FLIP Antibody (7A3D12) [NBP2-37359] - Western blot analysis using CFLAR mouse mAb against JURKAT (1), 3T3L1 (2) and RAJI (3) cell lysate.
Immunohistochemistry-Paraffin: FLIP Antibody (7A3D12) [NBP2-37359] - Analysis of esophagus cancer tissues using CFLAR mouse mAb with DAB staining.
Flow Cytometry: FLIP Antibody (7A3D12) [NBP2-37359] - Flow cytometric analysis of JURKAT cells using CFLAR mouse mAb (green) and negative control (red).
Western Blot: FLIP Antibody (7A3D12) [NBP2-37359] - Western blot analysis using CFLAR mAb against human CFLAR recombinant protein. (Expected MW is 42.9 kDa)
Immunohistochemistry-Paraffin: FLIP Antibody (7A3D12) [NBP2-37359] - Analysis of cervical cancer tissues using CFLAR mouse mAb with DAB staining.
ELISA: FLIP Antibody (7A3D12) [NBP2-37359] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
7A3D12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP2-37359-0.025ml
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FLIP Antibody (7A3D12) - BSA Free Summary

Immunogen
Purified recombinant fragment of human FLIP (AA: 100-251) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CFLAR
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for FLIP Antibody (7A3D12) - BSA Free

  • CASHFADD-like antiapoptotic molecule 1
  • CASP8 and FADD-like apoptosis regulator
  • CASP8AP1
  • Caspase homolog
  • Caspase-eight-related protein
  • Caspase-like apoptosis regulatory protein
  • caspase-related inducer of apoptosis
  • CASPER
  • CasperFLAME1
  • Cellular FLICE-like inhibitory protein
  • CFLAR
  • c-FLIPR
  • c-FLIPS
  • CLARPMACH-related inducer of toxicity
  • FADD-like apoptotic molecule
  • FLAME
  • FLAME-1
  • FLIP
  • I-FLICE
  • I-FLICEMRITc-FLIPFLIPc-FLIPL
  • Inhibitor of FLICE
  • usurpin beta
  • Usurpin

Background

Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CFLAR