Name: Fibroblast Activation Protein alpha/FAP Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF3715
Rating: 4.5 (2 reviews)

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, Simple Western, IHC, IP, Dual ISH-IHC, KO
Clonality	Polyclonal
Host	Sheep
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Biotin BAF3715

Catalog# & Formulation	Size	Price
Carrier Free AF3715-SP

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF3715

Fibroblast Activation Protein alpha/FAP Antibody [Unconjugated]

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Publications for Fibroblast Activation Protein alpha/FAP Antibody (AF3715)(46)

Reviews for Fibroblast Activation Protein alpha/FAP Antibody (AF3715) (2) 4.52

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Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP Leu26-Asp760 Accession # Q12884
Specificity	Detects human Fibroblast Activation Protein alpha /FAP in direct ELISAs and Western blots.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Sheep
Gene	FAP
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Publications using AF3715	Applications	Species
Di Carlo, E;Sorrentino, C; The multifaceted role of the stroma in the healthy prostate and prostate cancer Journal of translational medicine 2024-09-05 [PMID: 39238004] (Immunohistochemistry, Human)	Immunohistochemistry	Human
Lee, JY;Reyes, N;Woo, SH;Goel, S;Stratton, F;Kuang, C;Mansfield, AS;LaFave, LM;Peng, T; Senescent fibroblasts in the tumor stroma rewire lung cancer metabolism and plasticity bioRxiv : the preprint server for biology 2024-07-30 [PMID: 39131266] (Immunohistochemistry, Transgenic Mouse)	Immunohistochemistry	Transgenic Mouse
Oguri, N;Gi, T;Nakamura, E;Furukoji, E;Goto, H;Maekawa, K;Tsuji, AB;Nishii, R;Aman, M;Moriguchi-Goto, S;Sakae, T;Azuma, M;Yamashita, A; Expression of fibroblast activation protein-? in human deep vein thrombosis Thrombosis research 2024-06-25 [PMID: 38955058] (Immunocytochemistry, Human)	Immunocytochemistry	Human
Susanne N. Wijesinghe, Amel Badoume, Dominika E. Nanus, Archana Sharma‐Oates, Hussein Farah, Michelangelo Certo et al. Obesity defined molecular endotypes in the synovium of patients with osteoarthritis provides a rationale for therapeutic targeting of fibroblast subsets Clinical and Translational Medicine 2023-04-03 [PMID: 37006170]
Noriko Mori, Jiefu Jin, Balaji Krishnamachary, Yelena Mironchik, Flonné Wildes, Farhad Vesuna et al. Functional roles of FAP-alpha in metabolism, migration and invasion of human cancer cells Frontiers in Oncology 2023-03-01 [PMID: 36937451]
A Pappalardo, D Alvarez Ce, S Fang, AR Herschman, EY Jeon, KM Myers, JW Kysar, HE Abaci Engineering edgeless human skin with enhanced biomechanical properties Science Advances, 2023-01-27;9(4):eade2514. 2023-01-27 [PMID: 36706190] (IHC, Human)	IHC	Human
Tania Baltazar, Bo Jiang, Alejandra Moncayo, Jonathan Merola, Mohammad Z. Albanna, W. Mark Saltzman et al. 3D bioprinting of an implantable xeno‐free vascularized human skin graft Bioengineering & Translational Medicine 2023-01-01 [PMID: 36684084]
Aoyuan Fan, Genbin Wu, Jianfang Wang, Laiya Lu, Jingyi Wang, Hanjing Wei et al. Inhibition of fibroblast activation protein ameliorates cartilage matrix degradation and osteoarthritis progression Bone Research 2023-01-02 [PMID: 36588124]
B Li, Z Ding, O Calbay, Y Li, T Li, L Jin, S Huang FAP is critical for ovarian cancer cell survival by sustaining NF-kappaB activation through recruitment of PRKDC in lipid rafts Cancer Gene Therapy, 2022-12-09;0(0):. 2022-12-09 [PMID: 36494579] (IP, Mouse)	IP	Mouse
W Zeng, L Xiong, W Wu, S Li, J Liu, L Yang, L Lao, P Huang, M Zhang, H Chen, N Miao, Z Lin, Z Liu, X Yang, J Wang, P Wang, E Song, Y Yao, Y Nie, J Chen, D Huang CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype Oncogene, 2022-11-22;0(0):. 2022-11-22 [PMID: 36418470] (Flow Cytometry, IHC, Western Blot, Human)	Flow Cytometry, IHC, Western Blot	Human
Jiefu Jin, James D. Barnett, Balaji Krishnamachary, Yelena Mironchik, Catherine K. Luo, Hisataka Kobayashi et al. Evaluating near‐infrared photoimmunotherapy for targeting fibroblast activation protein‐ alpha expressing cells in vitro and in vivo Cancer Science 2023-01-01 [PMID: 36169301]
RG Marangoni, P Datta, A Paine, S Duemmel, MA Nuzzo, L Sherwood, J Varga, C Ritchlin, BD Korman Thy-1 plays a pathogenic role and is a potential biomarker for skin fibrosis in scleroderma JCI Insight, 2022-10-10;0(0):. 2022-10-10 [PMID: 36066980] (IHC, Mouse)	IHC	Mouse
M Lindgren, G Rask, J Jonsson, A Berglund, C Lundin, P Jonsson, I Ljuslinder, H Nyström Type IV Collagen in Human Colorectal Liver Metastases-Cellular Origin and a Circulating Biomarker Cancers, 2022-07-13;14(14):. 2022-07-13 [PMID: 35884455] (IHC, Human)	IHC	Human
Lingling Ou, Huaishan Wang, Hui Huang, Zhiyan Zhou, Qiang Lin, Yeye Guo et al. Preclinical platforms to study therapeutic efficacy of human gamma δ T cells Clinical and Translational Medicine 6/22/2022 [PMID: 35731974]
II Verginadis, H Avgousti, J Monslow, G Skoufos, F Chinga, K Kim, NM Leli, IV Karagounis, BI Bell, A Velalopoul, CS Salinas, VS Wu, Y Li, J Ye, DA Scott, AL Osterman, A Sengupta, A Weljie, M Huang, D Zhang, Y Fan, E Radaelli, JW Tobias, F Rambow, P Karras, JC Marine, X Xu, AG Hatzigeorg, S Ryeom, JA Diehl, SY Fuchs, E Puré, C Koumenis A stromal Integrated Stress Response activates perivascular cancer-associated fibroblasts to drive angiogenesis and tumour progression Nature Cell Biology, 2022-06-02;0(0):. 2022-06-02 [PMID: 35654839] (IHC, Mouse)	IHC	Mouse
Geni Ye, Maohua Huang, Yong Li, Jie Ouyang, Minfeng Chen, Qing Wen et al. The FAP alpha -activated prodrug Z-GP-DAVLBH inhibits the growth and pulmonary metastasis of osteosarcoma cells by suppressing the AXL pathway Acta Pharmaceutica Sinica B 2022-03-01 [PMID: 35530139]
Laura De Vargas Roditi, Andrea Jacobs, Jan H. Rueschoff, Pete Bankhead, Stéphane Chevrier, Hartland W. Jackson et al. Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer Cell Reports Medicine 2022-04-19 [PMID: 35492239]
Layla Mathieson, Richard A. O’Connor, Hazel Stewart, Paige Shaw, Kevin Dhaliwal, Gareth O. S. Williams et al. Fibroblast Activation Protein Specific Optical Imaging in Non-Small Cell Lung Cancer Frontiers in Oncology 2022-03-10 [PMID: 35359378]
Chivu-Economescu M, Necula LG, Matei L et al. Collagen Family and Other Matrix Remodeling Proteins Identified by Bioinformatics Analysis as Hub Genes Involved in Gastric Cancer Progression and Prognosis International journal of molecular sciences 3/16/2022 [PMID: 35328635]
JW Opzoomer, JE Anstee, I Dean, EJ Hill, I Bouybayoun, J Caron, T Muliaditan, P Gordon, D Sosnowska, R Nuamah, SE Pinder, T Ng, F Dazzi, S Kordasti, DR Withers, T Lawrence, JN Arnold Macrophages orchestrate the expansion of a proangiogenic perivascular niche during cancer progression Science Advances, 2021-11-03;7(45):eabg9518. 11/3/2021 [PMID: 34730997]
M Friedrich, M Pohin, MA Jackson, I Korsunsky, SJ Bullers, K Rue-Albrec, Z Christofor, D Sathananth, T Thomas, R Ravindran, R Tandon, RS Peres, H Sharpe, K Wei, GFM Watts, EH Mann, A Geremia, M Attar, Oxford IBD, Roche Fibr, S McCuaig, L Thomas, E Collantes, HH Uhlig, SN Sansom, A Easton, S Raychaudhu, SP Travis, FM Powrie IL-1-driven stromal-neutrophil interactions define a subset of patients with inflammatory bowel disease that does not respond to therapies Nature Medicine, 2021-10-21;0(0):. 2021-10-21 [PMID: 34675383] (IHC, Human)	IHC	Human
M Shimizu, YI Koma, H Sakamoto, S Tsukamoto, Y Kitamura, S Urakami, K Tanigawa, T Kodama, N Higashino, M Nishio, M Shigeoka, Y Kakeji, H Yokozaki Metallothionein 2A Expression in Cancer-Associated Fibroblasts and Cancer Cells Promotes Esophageal Squamous Cell Carcinoma Progression Cancers, 2021-09-10;13(18):. 2021-09-10 [PMID: 34572779] (Western Blot, Human)	Western Blot	Human
Richard A O’Connor, Vishwani Chauhan, Layla Mathieson, Helen Titmarsh, Lilian Koppensteiner, Irene Young et al. T cells drive negative feedback mechanisms in cancer associated fibroblasts, promoting expression of co-inhibitory ligands, CD73 and IL-27 in non-small cell lung cancer OncoImmunology 2021-07-22 [PMID: 34290905]
N Ganig, F Baenke, ML Thepkayson, K Lin, VS Rao, FC Wong, H Polster, M Schneider, D Helm, M Pecqueux, AM Seifert, L Seifert, J Weitz, NN Rahbari, C Kahlert Proteomic Analyses of Fibroblast- and Serum-Derived Exosomes Identify QSOX1 as a Marker for Non-invasive Detection of Colorectal Cancer Cancers, 2021-03-17;13(6):. 2021-03-17 [PMID: 33802764] (IHC, Western Blot, Human)	IHC, Western Blot	Human
LE Bruijn, BEWM van den Ak, CM van Rhijn, JF Hamming, JHN Lindeman Extreme Diversity of the Human Vascular Mesenchymal Cell Landscape J Am Heart Assoc, 2020-11-16;9(23):e017094. 2020-11-16 [PMID: 33190596] (ICC, IHC, Human)	ICC, IHC	Human
C Cho, R Mukherjee, AR Peck, Y Sun, N McBrearty, KV Katlinski, J Gui, PK Govindaraj, E Puré, H Rui, SY Fuchs Cancer-associated fibroblasts downregulate type I interferon receptor to stimulate intratumoral stromagenesis Oncogene, 2020-08-17;0(0):. 2020-08-17 [PMID: 32807917] (ICC, IHC, Human)	ICC, IHC	Human
Shenghua Zhan, Zhiju Liu, Min Zhang, Tianwei Guo, Qiuying Quan, Lili Huang et al. Overexpression of B7-H3 in alpha -SMA-Positive Fibroblasts Is Associated With Cancer Progression and Survival in Gastric Adenocarcinomas Frontiers in Oncology 2020-01-10 [PMID: 31998637]
N Shirasu, H Shibaguchi, H Yamada, M Kuroki, S Yasunaga Highly versatile cancer photoimmunotherapy using photosensitizer-conjugated avidin and biotin-conjugated targeting antibodies Cancer Cell Int., 2019-11-15;19(0):299. 2019-11-15 [PMID: 31787847] (Photoimmunotherapy, Human)	Photoimmunotherapy	Human
D Correa-Gal, D Jiang, S Christ, P Ramesh, H Ye, J Wannemache, S Kalgudde G, Q Yu, M Aichler, A Walch, U Mirastschi, T Volz, Y Rinkevich Patch repair of deep wounds by mobilized fascia Nature, 2019-11-27;0(0):. 2019-11-27 [PMID: 31776510] (IHC, Human)	IHC	Human
Johanna Wagner, Maria Anna Rapsomaniki, Stéphane Chevrier, Tobias Anzeneder, Claus Langwieder, August Dykgers et al. A Single-Cell Atlas of the Tumor and Immune Ecosystem of Human Breast Cancer Cell 2019-05-16 [PMID: 30982598]
TP Sandberg, MPME Stuart, J Oosting, RAEM Tollenaar, CFM Sier, WE Mesker Increased expression of cancer-associated fibroblast markers at the invasive front and its association with tumor-stroma ratio in colorectal cancer BMC Cancer, 2019-03-29;19(1):284. 3/29/2019 [PMID: 30922247]
N Higashino, YI Koma, M Hosono, N Takase, M Okamoto, H Kodaira, M Nishio, M Shigeoka, Y Kakeji, H Yokozaki Fibroblast activation protein-positive fibroblasts promote tumor progression through secretion of CCL2 and interleukin-6 in esophageal squamous cell carcinoma Lab. Invest., 2019-01-25;0(0):. 2019-01-25 [PMID: 30683902] (IHC, Human)	IHC	Human
T Muliaditan, J Caron, M Okesola, JW Opzoomer, P Kosti, M Georgouli, P Gordon, S Lall, DM Kuzeva, L Pedro, JD Shields, CE Gillett, SS Diebold, V Sanz-Moren, T Ng, E Hoste, JN Arnold Macrophages are exploited from an innate wound healing response to facilitate cancer metastasis Nat Commun, 2018-07-27;9(1):2951. 7/27/2018 [PMID: 30054470]
Ellen Puré, Rachel Blomberg Pro-tumorigenic roles of fibroblast activation protein in cancer: back to the basics Oncogene 2018-08-01 [PMID: 29720723]
MA Lakins, E Ghorani, H Munir, CP Martins, JD Shields Cancer-associated fibroblasts induce antigen-specific deletion of CD8+T Cells to protect tumour cells Nat Commun, 2018-03-05;9(1):948. 2018-03-05 [PMID: 29507342] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
XP Chai, GL Sun, YF Fang, LH Hu, X Liu, XW Zhang Tumor-targeting efficacy of a BF211 prodrug through hydrolysis by fibroblast activation protein-? Acta Pharmacol. Sin., 2017-11-09;0(0):. 2017-11-09 [PMID: 29119969] (Western Blot, Mouse)	Western Blot	Mouse
Jun Jia, Tracey A. Martin, Lin Ye, Lin Meng, Nan Xia, Wen G. Jiang et al. Fibroblast activation protein-alpha promotes the growth and migration of lung cancer cells via the PI3K and sonic hedgehog pathways International Journal of Molecular Medicine 2017-10-27 [PMID: 29115573]
A Lo, CP Li, EL Buza, R Blomberg, P Govindaraj, D Avery, J Monslow, M Hsiao, E Puré Fibroblast activation protein augments progression and metastasis of pancreatic ductal adenocarcinoma JCI Insight, 2017-10-05;2(19):. 2017-10-05 [PMID: 28978805] (IHC-P, Human)	IHC-P	Human
AS Nagaraja, RL Dood, G Armaiz-Pen, Y Kang, SY Wu, JK Allen, NB Jennings, LS Mangala, S Pradeep, Y Lyons, M Haemmerle, KM Gharpure, NC Sadaoui, C Rodriguez-, C Ivan, Y Wang, K Baggerly, P Ram, G Lopez-Bere, J Liu, SC Mok, L Cohen, SK Lutgendorf, SW Cole, AK Sood Adrenergic-mediated increases in INHBA drive CAF phenotype and collagens JCI Insight, 2017-08-17;2(16):. 2017-08-17 [PMID: 28814667] (IHC, Mouse)	IHC	Mouse
X Feng, Q Wang, Y Liao, X Zhou, Y Wang, W Liu, G Zhang A synthetic urinary probe-coated nanoparticles sensitive to fibroblast activation protein ? for solid tumor diagnosis Int J Nanomedicine, 2017-07-27;12(0):5359-5372. 2017-07-27 [PMID: 28794628] (Western Blot, Human, Mouse)	Western Blot	Human, Mouse
Meghna Waghray, Malica Yalamanchili, Michele Dziubinski, Mina Zeinali, Marguerite Erkkinen, Huibin Yang et al. GM-CSF Mediates Mesenchymal–Epithelial Cross-talk in Pancreatic Cancer Cancer Discovery 2016-08-01 [PMID: 27184426]
MH Fan, Q Zhu, HH Li, HJ Ra, S Majumdar, DL Gulick, JA Jerome, DH Madsen, M Christofid, DW Speicher, WW Bachovchin, C Feghali-Bo, E Puré Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in Mice J. Biol. Chem., 2015-12-09;291(15):8070-89. 2015-12-09 [PMID: 26663085] (IHC, Immunoprecipitation, Western Blot, Mouse)	IHC, Immunoprecipitation, Western Blot	Mouse
Shicheng Su, Qiyi Zhao, Chonghua He, Di Huang, Jiang Liu, Fei Chen et al. miR-142-5p and miR-130a-3p are regulated by IL-4 and IL-13 and control profibrogenic macrophage program Nature Communications 2015-10-05 [PMID: 26436920]
Kakarla S, Chow K, Mata M, Shaffer D, Song X, Wu M, Liu H, Wang L, Rowley D, Pfizenmaier K, Gottschalk S Antitumor effects of chimeric receptor engineered human T cells directed to tumor stroma. Mol Ther, 2013-06-04;21(8):1611-20. 2013-06-04 [PMID: 23732988] (CAR-T (Flow Cytometry), CAR-T (Flow Cytomety), Human)	CAR-T (Flow Cytometry), CAR-T (Flow Cytomety)	Human
Edward W. Roberts, Andrew Deonarine, James O. Jones, Alice E. Denton, Christine Feig, Scott K. Lyons et al. Depletion of stromal cells expressing fibroblast activation protein-alpha from skeletal muscle and bone marrow results in cachexia and anemia Journal of Experimental Medicine 2013-06-03 [PMID: 23712428]
Kraman M, Bambrough P, Arnold J, Roberts E, Magiera L, Jones J, Gopinathan A, Tuveson D, Fearon D Suppression of antitumor immunity by stromal cells expressing fibroblast activation protein-alpha. Science, 2010-11-05;330(6005):827-30. 2010-11-05 [PMID: 21051638] (Flow Cytometry, IHC, Mouse)	Flow Cytometry, IHC	Mouse
Show All 46 Publications. Collapse Publications.

	Mohamad Youness 08/01/2023
Application:	ICC
Species:	Human

	Santhosh Sivajothi 04/15/2019
Application:	IHC-P
Species:	Human

Gene Symbol	FAP
Uniprot	Q12884()