| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA(Det) |
| Clonality | Polyclonal |
| Host | Sheep |
| Conjugate | Biotin |
| Concentration | LYOPH |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Fibroblast Activation Protein alpha /FAP Leu26-Asp760 Accession # Q12884 |
| Specificity | Detects human Fibroblast Activation Protein alpha /FAP in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human DPPIV is observed. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Sheep |
| Gene | FAP |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | ELISA Capture: Human Fibroblast Activation Protein alpha/FAP Antibody (Catalog # MAB3715) ELISA Detection: Human Fibroblast Activation Protein alpha/FAP Biotinylated Antibody (Catalog # BAF3715) Standard:Recombinant Human Fibroblast Activation Protein alpha/FAP (Catalog # 3715-SE) |
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| Readout System | ||
| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
FAP (also known as seprase) is a Type II transmembrane serine protease that is structurally related to dipeptidyl peptidase IV (1). FAP has substrate specificity similar to dipeptidyl peptidase IV, which is specific for N-terminal Xaa-Pro sequences, but FAP is also an endopeptidase able to degrade gelatin and Type I collagen (2). The enzymatically active form of FAP is a dimer (3). FAP has a restricted tissue distribution. It is not detectable in normal tissues or resting fibroblasts, but is highly expressed on reactive stromal fibroblasts in epithelial cancers (4), in granulation tissue during wound healing, and in bone and soft tissue sarcomas (5). Because of its expression patterns and enzymatic activities, FAP is believed to play roles in tumor invasion, tissue remodeling, and wound repair.
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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