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Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free

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Western Blot: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Fibrinogen gamma chain.
Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western Blot: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732] - Analysis of extracts (35ug) from 9 different cell lines by using FGG monoclonal antibody (HepG2: human; HeLa: human; SVT2: ...read more
Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for ...read more
Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C ...read more
Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free [NBP2-70732] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, IHC
Clone
OTI2D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human FGG(NP_000500) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FGG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:400-4000
Theoretical MW
46.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Fibrinogen gamma chain Antibody (OTI2D2) - Azide and BSA Free

  • fibrinogen gamma chain
  • fibrinogen, gamma polypeptide

Background

The protein encoded by the Fibrinogen gamma chain gene is the gamma component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia. Alternative splicing results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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CD11b Expression, Leukocyte Adhesion and the Innate Immune System
What is CD11b?CD11b is an integrin family member which pairs with CD18 to form the CR3 heterodimer. CD11b is expressed on the surface of many leukocytes including monocytes, neutrophils, natural killer cells, granulocytes and macrophages, as well as o...  Read full blog post.

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Bioinformatics

Gene Symbol FGG