Home » FGFR3 » FGFR3 Antibodies » FGFR3 Antibody (136334) [Unconjugated]
Novus Biologicals products are now on bio-techne.com

FGFR3 Antibody (136334) [Unconjugated]

 (4 Publications)
 (1 Review)
 See All Related
Submit a Review

Images

 
(
Enlarge)
TAB cells modulate melanoma cells to express FGFR-3 and its ligand FGF-2 for tumor stroma-tumor cell cross-talk. a WM3749 co-cultured (72 h) with TAB cells (red bar) show increased FGFR-3 mRNA expression when compared with tumors only (open bar) or co-cultured with NB cells (blue bar). b Lysates obtained from pools of melanomas co-cultured (72–120 h) with NB- or TAB cells were probed in western blot with anti-FGFR-3 antibody (left panel), results expressed as relative intensity after beta -actin normalization (right panel). c Melanoma cells co-cultured with TAB cells (72 h) show increased phospho-FGFR-3 expression (right panel; immunofluorescence assays) when compared with melanoma cells alone (left panel) or melanoma cells co-cultured with NB cells (middle panel), scale bars: 40 μm, images captured by Nikon inverted microscope. d Melanoma cells co-cultured with TAB cells (red bar) show increased FGF-2 mRNA expression when compared with melanoma cells alone (open bar) or melanoma cells co-cultured with NB cells (blue bar). e 451Lu and WM989treated with IGF-1 (25 ng/ml/daily for 5 days; red bar) show increased FGFR-3 expression when compared with untreated controls (blue bar), flow cytometry results expressed as net % expression of control antibody. IGF-1 treated melanoma cells (red bars) show higher expression of FGFR-3 compared with untreated cells (blue bars). Bar represents mean + SD of replicate samples. f NB cells treated with FGF-2 (10 ng/ml/daily for 4 days; red bar) show high IGF-1 mRNA expression relative to untreated NB cells (blue bar). g 451Lu and WM989 co-cultured (72 h) with TAB cells in the presence of an anti-IGF-1 neutralizing antibody (10 μg/ml) show decreased FGFR-3 mRNA expression in tumor cells (blue bar) when compared with controls (red bar). h TAB cells co-cultured (72 h) with 451Lu and WM989 in the presence of an anti-FGF-2 neutralizing antibody (1 μg/ml) show decreased IGF-1 mRNA expression in B cells (blue bar) when compared with controls (red bar).Experiments in a, d and f–h were performed using qPCR. In Figures a, d–h, bars represent mean + SE of duplicate samples and are representative of at least two independent experiments. i Summary of cross-talk between melanoma and B cells: FGF-2 is constitutively expressed by tumor cells, released into the microenvironment to bind FGFR-3 on the B cells, activated B cells express increased levels of pro-inflammatory cytokines. IGF-1 released by TAB cells modulates tumor cells to increase their growth, heterogeneity and therapy resistance Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28928360), licensed under a CC-BY license. Not internally tested by R&D Systems.
TAB cells modulate melanoma cells to express FGFR-3 and its ligand FGF-2 for tumor stroma-tumor cell cross-talk. a WM3749 co-cultured (72 h) with TAB cells (red bar) show increased FGFR-3 mRNA expression when compared ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready
Clone
136334
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB766U-100UG
Alexa Fluor 405
FAB766V-100UG
Alexa Fluor 488
FAB766G-100UG
Alexa Fluor 532
FAB766X
Alexa Fluor 594
FAB766T-100UG
Alexa Fluor 647
FAB766R-100UG
Alexa Fluor 700
FAB766N-100UG
Alexa Fluor 750
FAB766S-100UG
Allophycocyanin
FAB766A
Allophycocyanin/Cy7
FAB766APCCY7
Biotin
FAB766B
CoraFluor 1
FAB766CL1
DyLight 350
FAB766D
DyLight 405
FAB766E
DyLight 488
FAB766K
DyLight 550
FAB766L
DyLight 594
FAB766M
DyLight 650
FAB766W
DyLight 680
FAB766Y
DyLight 755
FAB766Z
Fluorescein
FAB766F
HRP
FAB766H
Janelia Fluor 525
FAB766JF525
Janelia Fluor 549
FAB766I
Janelia Fluor 646
FAB766J
Janelia Fluor 669
FAB766JF669
PE/Atto594
FAB766PEATT594
PE/Cy5.5
FAB766PECY55
PE/Cy7
FAB766PECY7
Phycoerythrin
FAB766P
mFluor Violet 450 SE
FAB766MFV450
mFluor Violet 500 SE
FAB766MFV500
mFluor Violet 610 SE
FAB766MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB766F
Carrier Free
FAB766P
Carrier Free
FAB766A
Carrier Free
MAB766-SP
Carrier Free
MAB766-500
Carrier Free
FAB766V-100UG
Carrier Free
FAB766U-100UG
Carrier Free
FAB766T-100UG
Carrier Free
FAB766S-100UG
Carrier Free
FAB766R-100UG
Carrier Free
FAB766N-100UG
Carrier Free
FAB766G-100UG
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

FGFR3 Antibody (136334) [Unconjugated] Summary

Additional Information
Clone 136334 was used by HLDA to establish CD designation
Immunogen
Pool of NS0-derived recombinant human FGF R3 alpha (IIIb) and Sf21-derived FGF R3 alpha (IIIc)
Specificity
Detects the IIIb and IIIc isoforms of human FGF R3 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FGFR3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB766 in the following applications:
Publications
Read Publications using
MAB766 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FGFR3 Antibody (136334) [Unconjugated]

  • CD333
  • CEK
  • EC 2.7.10
  • FGF R3
  • FGFR3
  • fibroblast growth factor receptor 3
  • HSFGFR3EX
  • JTK4
  • JTK4thanatophoric dwarfism

Background

Fibroblast Growth Factor Receptor 3 (FGF R3) is a type I transmembrane tyrosine kinase receptor that binds FGF ligands along with heparin or heparin sulfate proteoglycans as co‑factors. A segment of the membrane proximal Ig-like domain can be encoded by two different exons resulting in (IIIb) or (IIIc) isoforms. The IIIb or IIIc isoforms recognize FGF -1, -2, -4, -8b, -8e, -8f, -9, and -17b. FGF R3 plays a role in skeletal, brain, lung, intestine, kidney, and skin development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

FGFR2 Antibody
(1G3)
H00002263-M01
Immunohistochemistry-Paraffin FGFR2 Antibody (1G3)
Western Blot FGFR2 Antibody (1G3)
Sandwich ELISA FGFR2 Antibody (1G3)
(13 Publications)
Species: Bv, Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
FGFR1 Antibody
(M19B2) - BSA Free
NB600-1287
Flow Cytometry FGFR1 Antibody (M19B2) - BSA Free
Flow Cytometry FGFR1 Antibody (M19B2) - BSA Free
Western Blot FGFR1 Antibody (M19B2) - BSA Free
(1 Review)
(7 Publications)
Species: Ca, Hu, Mu, Rb, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, WB
FGF
basic/FGF2/bFGF [Unco
233-FB
FGF basic/FGF2/bFGF [Unconjugated]
(550 Publications)
Species: Hu
Applications: BA
FGF acidic/FGF1
Antibody [Unconjugate
AF232
Immunohistochemistry FGF acidic/FGF1 Antibody [Unconjugated]
Simple Western FGF acidic/FGF1 Antibody [Unconjugated]
Western Blot FGF acidic/FGF1 Antibody [Unconjugated]
(13 Publications)
Species: Hu
Applications: IHC, Neut, Simple Western, WB
FGFR4 Antibody
(240929) [Unconjugate
MAB6852
Immunocytochemistry FGFR4 Antibody (240929) [Unconjugated]
Immunohistochemistry FGFR4 Antibody (240929) [Unconjugated]
Western Blot FGFR4 Antibody (240929) [Unconjugated]
(2 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, ICC, IHC, WB
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(42 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
Immunohistochemistry p19ARF/CDKN2A Antibody - BSA Free
Western Blot p19ARF/CDKN2A Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence p19ARF/CDKN2A Antibody - BSA Free
(1 Review)
(48 Publications)
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
FGF-9
[Unconjugated]
273-F9
Bioactivity FGF-9 [Unconjugated]
(47 Publications)
Species: Hu
Applications: BA
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
Cyclin D1 Antibody
(SPM587)
NBP2-32840
Western Blot Cyclin D1 Antibody (SPM587)
Flow Cytometry Cyclin D1 Antibody (SPM587)
Flow Cytometry Cyclin D1 Antibody (SPM587)
(1 Review)
(9 Publications)
Species: Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
WHSC1 Antibody
(CL1063)
NBP2-52963
Western Blot WHSC1 Antibody (CL1063)
Immunohistochemistry-Paraffin WHSC1 Antibody (CL1063)
Immunocytochemistry/ Immunofluorescence WHSC1 Antibody (CL1063)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin p21/CIP1/CDKN1A Antibody (WA-1)
(24 Publications)
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
FGF-13 Antibody
NBP2-16471
Immunohistochemistry-Paraffin FGF-13 Antibody
Western Blot FGF-13 Antibody
Immunohistochemistry-Paraffin FGF-13 Antibody
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
FGF-3
[Unconjugated]
1206-F3
FGF-3 [Unconjugated]
(14 Publications)
Species: Hu
Applications: BA
KGF/FGF-7
[Unconjugated]
251-KG
Bioactivity KGF/FGF-7 [Unconjugated]
Bioactivity KGF/FGF-7 [Unconjugated]
(85 Publications)
Species: Hu
Applications: BA
Twist-1 Antibody
(10E4E6) - BSA Free
NBP2-37364
Flow Cytometry Twist-1 Antibody (10E4E6) - BSA Free
Flow (Intracellular) Twist-1 Antibody (10E4E6) - BSA Free
Flow Cytometry Twist-1 Antibody (10E4E6) - BSA Free
(1 Review)
(9 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, KD, WB
FGF-8
[Unconjugated]
423-F8
SDS-PAGE FGF-8 [Unconjugated]
Bioactivity FGF-8 [Unconjugated]
Cell Culture FGF-8 [Unconjugated]
(1 Review)
(63 Publications)
Species: Hu, Mu
Applications: BA
PTHLH/PTHrP
Antibody (677939) [Un ...
MAB6734
Immunohistochemistry PTHLH/PTHrP Antibody (677939) [Unconjugated]
(2 Publications)
Species: Hu
Applications: IHC
FGFR3 Antibody
(136334) [Unconjugate
MAB766
FGFR3 Antibody (136334) [Unconjugated]
Species: Hu
Applications: WB, Flow, CyTOF-ready

Publications for FGFR3 Antibody (MAB766)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, Western Blot.


Filter By Application
Flow Cytometry
(3)
Western Blot
(1)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 4 of 4.
Publications using MAB766 Applications Species
Jakl, V;Popp, T;Haupt, J;Port, M;Roesler, R;Wiese, S;Friemert, B;Rojewski, MT;Schrezenmeier, H; Effect of Expansion Media on Functional Characteristics of Bone Marrow-Derived Mesenchymal Stromal Cells Cells 2023-08-19 [PMID: 37626914] (Flow Cytometry, Human) Flow Cytometry Human
BW Stringer, BW Day, RCJ D'Souza, PR Jamieson, KS Ensbey, ZC Bruce, YC Lim, K Goasdoué, C Offenhäuse, S Akgül, S Allan, T Robertson, P Lucas, G Tollesson, S Campbell, C Winter, H Do, A Dobrovic, PL Inglis, RL Jeffree, TG Johns, AW Boyd A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma Sci Rep, 2019-03-20;9(1):4902. 2019-03-20 [PMID: 30894629] (Flow Cytometry, Human) Flow Cytometry Human
R Somasundar, G Zhang, M Fukunaga-K, M Perego, C Krepler, X Xu, C Wagner, D Hristova, J Zhang, T Tian, Z Wei, Q Liu, K Garg, J Griss, R Hards, M Maurer, C Hafner, M Mayerhöfer, G Karanikas, A Jalili, V Bauer-Pohl, F Weihsengru, K Rappersber, J Koller, R Lang, C Hudgens, G Chen, M Tetzlaff, L Wu, DT Frederick, RA Scolyer, GV Long, M Damle, C Ellingswor, L Grinman, H Choi, BJ Gavin, M Dunagin, A Raj, N Scholler, L Gross, M Beqiri, K Bennett, I Watson, H Schaider, MA Davies, J Wargo, BJ Czerniecki, L Schuchter, D Herlyn, K Flaherty, M Herlyn, SN Wagner Tumor-associated B-cells induce tumor heterogeneity and therapy resistance Nat Commun, 2017-09-19;8(1):607. 2017-09-19 [PMID: 28928360] (Western Blot, Human) Western Blot Human
Stewart AK, Chang H, Trudel S Diagnostic evaluation of t(4;14) in multiple myeloma and evidence for clonal evolution. Leukemia, 2007-06-14;21(11):2358-9. 2007-06-14 [PMID: 17568814] (Flow Cytometry, Human) Flow Cytometry Human

Review for FGFR3 Antibody (MAB766) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB766:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry FGFR3 MAB766
Enlarge 		4
reviewed by:
Natalie Erdmann
 ICC Human 01/28/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedKMS11, KMS18 and RPMI8226 (multiple myeloma) cell lines
SpeciesHuman
LotGGC081601A

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FGFR3 Antibody (MAB766) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional FGFR3 Products

Blogs on FGFR3

There are no specific blogs for FGFR3, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0
Immunocytochemistry FGFR3 MAB766
Enlarge
 Natalie Erdmann
01/28/2016
Application: ICC
Species: Human
read this review

Bioinformatics

Gene Symbol FGFR3