FGF14 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

The FGF14 gene codes for a fibroblast growth factor 14 protein that in isoform 1 is 247 amino acids long at 27 kDA and in isoform 2 is 252 amino acids long at 28 kDA. This gene is a member of the fibroblast growth factor (FGF) family so is thought to participate in nervous system development and function. FGF14 is involved in regulation of actin cytoskeleton, MAPK signaling, mitochondrial apoptosis, paxillin pathways, and nuclear receptor activation by vitamin-A. It interacts with genes EGFR, FGFR1, FGFR2, FGFR3, and FGFR4. FGF14 is associated with pancreatitis, gingivitis, breast cancer, ladd syndrome, ataxia, cerebritis, retinitis, neuronitis, and adenocarcinoma.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBP2-07910

• FGF14 Antibodies
• FGF14 Lysates
• FGF14 Proteins