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Fc gamma RIII (CD16) Antibody (245536) [Unconjugated]

 (6 Publications)
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Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human Fc gamma RIII A/B (CD16a/b) Monoclonal Antibody (Catalog # MAB2546) or (B) Mouse IgG2A isotype control antibody (MAB003) followed by Goat anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol.
Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human Fc gamma RIII A/B (CD16a/b) Monoclonal Antibody (Catalog # MAB2546) or (B) Mouse IgG2A isotype control antibody (MAB003) followed ...read more
Anti-ULBP3 monoclonal antibody enhances the activity of NK cells from cancer patients via ADCC.ULBP3 expression and the percentage of infiltrating NK cells in tumor tissues from 10 patients (4 colorectal cancer, 3 lung ...read more
Anti-ULBP3 monoclonal antibody enhances the activity of NK cells from cancer patients via ADCC.ULBP3 expression and the percentage of infiltrating NK cells in tumor tissues from 10 patients (4 colorectal cancer, 3 lung ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
245536
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB2546U-100UG
Alexa Fluor 405
FAB2546V-100UG
Alexa Fluor 488
FAB2546G-100UG
Alexa Fluor 532
FAB2546X
Alexa Fluor 594
FAB2546T-100UG
Alexa Fluor 647
FAB2546R-100UG
Alexa Fluor 750
FAB2546S-100UG
Allophycocyanin
FAB2546A-025
Allophycocyanin
FAB2546A-100
Allophycocyanin/Cy7
FAB2546APCCY7
Biotin
FAB2546B-025
CoraFluor 1
FAB2546CL1
DyLight 350
FAB2546D
DyLight 405
FAB2546E
DyLight 488
FAB2546K
DyLight 550
FAB2546L
DyLight 594
FAB2546M
DyLight 650
FAB2546W
DyLight 680
FAB2546Y
DyLight 755
FAB2546Z
Fluorescein
FAB2546F-025
Fluorescein
FAB2546F-100
HRP
FAB2546H
Janelia Fluor 525
FAB2546JF525
Janelia Fluor 549
FAB2546I
Janelia Fluor 646
FAB2546J
Janelia Fluor 669
FAB2546JF669
PE/Atto594
FAB2546PEATT594
PE/Cy5.5
FAB2546PECY55
PE/Cy7
FAB2546PECY7
PerCP (Peridinin-chlorophyll Protein Complex)
FAB2546C-100
PerCP (Peridinin-chlorophyll Protein Complex)
FAB2546C-025
Phycoerythrin
FAB2546P-100
Phycoerythrin
FAB2546P-025
mFluor Violet 450 SE
FAB2546MFV450
mFluor Violet 500 SE
FAB2546MFV500
mFluor Violet 610 SE
FAB2546MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB2546R-100UG
Carrier Free
FAB2546S-100UG
Carrier Free
FAB2546T-100UG
Carrier Free
FAB2546U-100UG
Carrier Free
FAB2546V-100UG
Carrier Free
FAB2546B-025
Carrier Free
MAB2546-500
Carrier Free
MAB2546-SP
Carrier Free
FAB2546C-025
Carrier Free
FAB2546A-100
Carrier Free
FAB2546A-025
Carrier Free
FAB2546P-100
Carrier Free
FAB2546P-025
Carrier Free
FAB2546F-100
Carrier Free
FAB2546F-025
Carrier Free
FAB2546C-100
Carrier Free
FAB2546G-100UG
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Fc gamma RIII (CD16) Antibody (245536) [Unconjugated] Summary

Immunogen
Raised against Fc gamma RIII (CD16).
Source
N/A
Clonality
Monoclonal
Host
Mouse
Gene
FCGR3A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
Publications
Read Publications using
MAB2546 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fc gamma RIII (CD16) Antibody (245536) [Unconjugated]

  • CD16
  • CD16A
  • Fc fragment of IgG receptor IIIa
  • Fc gamma RIII
  • FCG3
  • FCGR3
  • FcgRIII
  • FCR-10
  • FCRIII
  • FCRIIIA
  • IGFR3
  • IMD20

Background

Fc gamma RIIIA and B are low affinity receptors for polyvalent immune-complexed IgG. Fc gamma RIIIA is a transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages. Fc gamma RIIIB is a non-activating GPI-linked decoy receptor expressed on neutrophils and eosinophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fc gamma RIII (CD16) Antibody (MAB2546)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Flow Cytometry, Functional Assay, ICC, Immunoprecipitation.


Filter By Application
Flow Cytometry
(4)
Functional Assay
(1)
ICC
(1)
Immunoprecipitation
(1)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB2546 Applications Species
KO Dixon, J O'Flynn, N Klar-Moham, MR Daha, C van Kooten Properdin and factor H production by human dendritic cells modulates their T-cell stimulatory capacity and is regulated by IFN-? Eur. J. Immunol, 2017-03-01;0(0):. 2017-03-01 [PMID: 28105653] (Flow Cytometry, Human) Flow Cytometry Human
Evidence for chemokine synergy during neutrophil migration in ARDS Thorax, 2016-08-05;0(0):. 2016-08-05 [PMID: 27496101] (Flow Cytometry, Human) Flow Cytometry Human
Chauhan A, Moore T, Bi Y, Chen C FcgammaRIIIa-Syk Co-signal Modulates CD4+ T-cell Response and Up-regulates Toll-like Receptor (TLR) Expression. J Biol Chem, 2015-11-18;291(3):1368-86. 2015-11-18 [PMID: 26582197] (Flow Cytometry, Human) Flow Cytometry Human
Chauhan A, Chen C, Moore T, DiPaolo R Induced expression of FcgammaRIIIa (CD16a) on CD4+ T cells triggers generation of IFN-gammahigh subset. J Biol Chem, 2015-01-02;290(8):5127-40. 2015-01-02 [PMID: 25556651] (Flow Cytometry, Human) Flow Cytometry Human
Mou , Xiao, Zhou , Yuepeng, Jiang , Peng, Zhou , Tong, Jiang , Qian, Xu , Chengche, Liu , Hongli, Zheng , Tingting, Yuan , Guoyue, Zhang , Yanyun, Chen , Deyu, Mao , Chaoming The regulatory effect of UL-16 binding protein-3 expression on the cytotoxicity of NK cells in cancer patients. Sci Rep, 2014-08-20;4(0):6138. 2014-08-20 [PMID: 25138242] (Functional Assay, Human) Functional Assay Human
Chauhan AK, Moore TL Immune complexes and late complement proteins trigger activation of Syk tyrosine kinase in human CD4(+) T cells. Clin. Exp. Immunol., 2012-02-01;167(2):235-45. 2012-02-01 [PMID: 22235999] (ICC, Immunoprecipitation, Human) ICC, Immunoprecipitation Human

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Bioinformatics

Gene Symbol FCGR3A
Uniprot