Home » Fc gamma RIIA/CD32a » Fc gamma RIIA/CD32a Antibodies » Fc gamma RIIA/CD32a Antibody (8.7) [Janelia Fluor® 669]
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Fc gamma RIIA/CD32a Antibody (8.7) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, CyTOF-ready, ICC/IF
Clone
8.7
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47831AF350
Alexa Fluor 405
NBP2-47831AF405
Alexa Fluor 488
NBP2-47831AF488
Alexa Fluor 532
NBP2-47831AF532
Alexa Fluor 594
NBP2-47831AF594
Alexa Fluor 647
NBP2-47831AF647
Alexa Fluor 700
NBP2-47831AF700
Alexa Fluor 750
NBP2-47831AF750
Allophycocyanin
NBP2-47831APC
Allophycocyanin/Cy7
NBP2-47831APCCY7
Biotin
NBP2-47831B-100ul
Biotin
NBP2-47831B
CoraFluor 1
NBP2-47831CL1
DyLight 350
NBP2-47831UV
DyLight 405
NBP2-47831V
DyLight 488
NBP2-47831G
DyLight 550
NBP2-47831R
DyLight 594
NBP2-47831DL594
DyLight 650
NBP2-47831C
DyLight 680
NBP2-47831FR
DyLight 755
NBP2-47831IR
FITC
NBP2-47831F
Janelia Fluor 525
NBP2-47831JF525
Janelia Fluor 549
NBP2-47831JF549
Janelia Fluor 585
NBP2-47831JF585
Janelia Fluor 635
NBP2-47831JF635
Janelia Fluor 646
NBP2-47831JF646
PE
NBP2-47831PE
PE/Atto594
NBP2-47831PEATT594
PE/Cy5.5
NBP2-47831PECY55
PE/Cy7
NBP2-47831PECY7
PerCP
NBP2-47831PCP
Unconjugated
NBP2-44722-0.1mg
mFluor Violet 450 SE
NBP2-47831MFV450
mFluor Violet 500 SE
NBP2-47831MFV500
mFluor Violet 610 SE
NBP2-47831MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44722-0.1mg
Azide and BSA Free
NBP2-47831-0.1mg
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Fc gamma RIIA/CD32a Antibody (8.7) [Janelia Fluor® 669] Summary

Immunogen
K562 and FcgRII+L cells
Localization
Cell Surface
Specificity
This monoclonal antibody reacts with a CD32 (FcgRII) epitope distinct from that defined by monoclonal antibody 8.26 and the epitope overlaps with that of monoclonal antibody 7.30 (cluster 4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FCGR2A
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Fc gamma RIIA/CD32a Antibody (8.7) [Janelia Fluor® 669]

  • CD32 antigen
  • CD32a
  • CD32MGC23887
  • CDw32fc-gamma-RIIa
  • Fc fragment of IgG, low affinity IIa, receptor (CD32)
  • Fc fragment of IgG, low affinity IIa, receptor for (CD32)
  • Fc gamma RIIA
  • FCG2
  • Fc-gamma RII-a
  • Fc-gamma-RIIa
  • FcGR
  • FCGR2
  • FCGR2A
  • FCGR2A1
  • FcgRIIA
  • FCRIIA
  • fcRII-a
  • IGFR2MGC30032
  • IgG Fc receptor II-a
  • Immunoglobulin G Fc receptor II
  • low affinity immunoglobulin gamma Fc region receptor II-a

Background

The FCGR2A gene encodes a low affinity immunoglobulin gamma Fc region receptor II-a protein that in isoform 1 is 317 amino acids long at 35 kDA and at isoform 2 is 316 amino acids long at nearly 35 kDA. These proteins are found on the surface of many immune system response cells that act as a low affinity receptor through encouraging responses against pathogens and soluble antigens. Additionally, it functions to initiate phagocytosis of opsonized antigens. FCGR2A participates in Fc-GammaR pathway, osteoclast differentiation, signaling events controlled by PTP1B, and NFAT in immune response. It is known to interact with genes SYK, FYN, HCK, LGALS3, and PIK3R1. FCGR2A is linked to lupus, thrombocytopenia, b-cell lymphomas, otitis media, adult-onset still's disease, guillain-barre syndrome, asymptomatic dengue, severe acute respiratory syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-47831JF669
Fc gamma RIIA/CD32a Antibody (8.7) [Janelia Fluor® 669]
Species: Hu
Applications: Flow, ICC/IF, CyTOF-ready, ICC/IF

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Fc gamma RIIA/CD32a Antibody (NBP2-47831JF669). (Showing 1 - 1 of 1 FAQ).

  1. Does any of your FCGR2A antibodies (19C10, 6D11, 13G9) cross react to FCGR2B?
    • For these antibodies, we have used the full-length protein FCGR2A (NP_067674) as the immunogen and we have not mapped the epitope that is detected with each Ab yet. I did an alignment of these two proteins and they are highly conserved thus it seems likely that there will be cross-reactivity.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol FCGR2A