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Fc gamma RIIA/CD32a Antibody (7.3) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, CyTOF-ready, ICC/IF
Clone
7.3
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47830AF350
Alexa Fluor 405
NBP2-47830AF405
Alexa Fluor 488
NBP2-47830AF488
Alexa Fluor 532
NBP2-47830AF532
Alexa Fluor 594
NBP2-47830AF594
Alexa Fluor 647
NBP2-47830AF647
Alexa Fluor 700
NBP2-47830AF700
Alexa Fluor 750
NBP2-47830AF750
Allophycocyanin
NBP2-47830APC
Allophycocyanin/Cy7
NBP2-47830APCCY7
Biotin
NBP2-47830B-100ul
Biotin
NBP2-47830B
DyLight 350
NBP2-47830UV
DyLight 405
NBP2-47830V
DyLight 488
NBP2-47830G
DyLight 550
NBP2-47830R
DyLight 594
NBP2-47830DL594
DyLight 650
NBP2-47830C
DyLight 680
NBP2-47830FR
DyLight 755
NBP2-47830IR
FITC
NBP2-47830F
Janelia Fluor 525
NBP2-47830JF525
Janelia Fluor 549
NBP2-47830JF549
Janelia Fluor 585
NBP2-47830JF585
Janelia Fluor 635
NBP2-47830JF635
Janelia Fluor 646
NBP2-47830JF646
Janelia Fluor 669
NBP2-47830JF669
PE
NBP2-47830PE
PE/Atto594
NBP2-47830PEATT594
PE/Cy5.5
NBP2-47830PECY55
PE/Cy7
NBP2-47830PECY7
PerCP
NBP2-47830PCP
Unconjugated
NBP2-44721-0.1mg
mFluor Violet 450 SE
NBP2-47830MFV450
mFluor Violet 500 SE
NBP2-47830MFV500
mFluor Violet 610 SE
NBP2-47830MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44721-0.1mg
Azide and BSA Free
NBP2-47830-0.1mg
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Fc gamma RIIA/CD32a Antibody (7.3) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
K562 and FcgRII+L cells
Localization
Cell Surface
Specificity
This monoclonal antibody reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (FcRII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FCGR2A
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Fc gamma RIIA/CD32a Antibody (7.3) [CoraFluor™ 1]

  • CD32 antigen
  • CD32a
  • CD32MGC23887
  • CDw32fc-gamma-RIIa
  • Fc fragment of IgG, low affinity IIa, receptor (CD32)
  • Fc fragment of IgG, low affinity IIa, receptor for (CD32)
  • Fc gamma RIIA
  • FCG2
  • Fc-gamma RII-a
  • Fc-gamma-RIIa
  • FcGR
  • FCGR2
  • FCGR2A
  • FCGR2A1
  • FcgRIIA
  • FCRIIA
  • fcRII-a
  • IGFR2MGC30032
  • IgG Fc receptor II-a
  • Immunoglobulin G Fc receptor II
  • low affinity immunoglobulin gamma Fc region receptor II-a

Background

The FCGR2A gene encodes a low affinity immunoglobulin gamma Fc region receptor II-a protein that in isoform 1 is 317 amino acids long at 35 kDA and at isoform 2 is 316 amino acids long at nearly 35 kDA. These proteins are found on the surface of many immune system response cells that act as a low affinity receptor through encouraging responses against pathogens and soluble antigens. Additionally, it functions to initiate phagocytosis of opsonized antigens. FCGR2A participates in Fc-GammaR pathway, osteoclast differentiation, signaling events controlled by PTP1B, and NFAT in immune response. It is known to interact with genes SYK, FYN, HCK, LGALS3, and PIK3R1. FCGR2A is linked to lupus, thrombocytopenia, b-cell lymphomas, otitis media, adult-onset still's disease, guillain-barre syndrome, asymptomatic dengue, severe acute respiratory syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Fc gamma
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NBP2-47830CL1
Fc gamma RIIA/CD32a Antibody (7.3) [CoraFluor™ 1]
Species: Hu
Applications: Flow, ICC/IF, CyTOF-ready, ICC/IF

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Fc gamma RIIA/CD32a Antibody (NBP2-47830CL1). (Showing 1 - 1 of 1 FAQ).

  1. Does any of your FCGR2A antibodies (19C10, 6D11, 13G9) cross react to FCGR2B?
    • For these antibodies, we have used the full-length protein FCGR2A (NP_067674) as the immunogen and we have not mapped the epitope that is detected with each Ab yet. I did an alignment of these two proteins and they are highly conserved thus it seems likely that there will be cross-reactivity.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol FCGR2A