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FBXW7/Cdc4 Antibody (OTI6F5) - Azide and BSA Free

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Western Blot: FBXW7/Cdc4 Antibody (OTI6F5) - Azide and BSA Free [NBP2-71995] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXW7.
Western Blot: FBXW7/Cdc4 Antibody (OTI6F5) - Azide and BSA Free [NBP2-71995] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXW7.
Immunohistochemistry: FBXW7/Cdc4 Antibody (OTI6F5) - Azide and BSA Free [NBP2-71995] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: FBXW7/Cdc4 Antibody (OTI6F5) - Azide and BSA Free [NBP2-71995] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: FBXW7/Cdc4 Antibody (OTI6F5) - Azide and BSA Free [NBP2-71995] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: FBXW7/Cdc4 Antibody (OTI6F5) - Azide and BSA Free [NBP2-71995] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: FBXW7/Cdc4 Antibody (OTI6F5) - Azide and BSA Free [NBP2-71995] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: FBXW7/Cdc4 Antibody (OTI6F5) - Azide and BSA Free [NBP2-71995] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: FBXW7/Cdc4 Antibody (OTI6F5) - Azide and BSA Free [NBP2-71995] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: FBXW7/Cdc4 Antibody (OTI6F5) - Azide and BSA Free [NBP2-71995] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: FBXW7/Cdc4 Antibody (OTI6F5) - Azide and BSA Free [NBP2-71995] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI6F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71995AF350
Alexa Fluor 405
NBP2-71995AF405
Alexa Fluor 488
NBP2-71995AF488
Alexa Fluor 532
NBP2-71995AF532
Alexa Fluor 594
NBP2-71995AF594
Alexa Fluor 647
NBP2-71995AF647
Alexa Fluor 700
NBP2-71995AF700
Alexa Fluor 750
NBP2-71995AF750
Allophycocyanin
NBP2-71995APC
Biotin
NBP2-71995B
CoraFluor 1
NBP2-71995CL1
DyLight 350
NBP2-71995UV
DyLight 405
NBP2-71995V
DyLight 488
NBP2-71995G
DyLight 550
NBP2-71995R
DyLight 594
NBP2-71995DL594
DyLight 650
NBP2-71995C
DyLight 680
NBP2-71995FR
DyLight 755
NBP2-71995IR
FITC
NBP2-71995F
HRP
NBP2-71995H
Janelia Fluor 525
NBP2-71995JF525
Janelia Fluor 549
NBP2-71995JF549
Janelia Fluor 585
NBP2-71995JF585
Janelia Fluor 635
NBP2-71995JF635
Janelia Fluor 646
NBP2-71995JF646
Janelia Fluor 669
NBP2-71995JF669
PE
NBP2-71995PE
PerCP
NBP2-71995PCP
Unconjugated
NBP2-45623
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45623
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FBXW7/Cdc4 Antibody (OTI6F5) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 1-354 of human FBXW7 (NP_361014) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
FBXW7
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
Theoretical MW
79.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for FBXW7/Cdc4 Antibody (OTI6F5) - Azide and BSA Free

  • AGO
  • AGOFBXO30
  • Archipelago Homolog
  • archipelago, Drosophila, homolog of
  • Cdc4
  • CDC4FLJ16457
  • F-box and WD repeat domain containing 7
  • F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila)
  • F-box and WD-40 domain-containing protein 7
  • F-box protein FBW7
  • F-box protein FBX30
  • F-box protein SEL-10
  • F-box/WD repeat-containing protein 7
  • FBW6
  • Fbw7
  • FBW7hCdc4
  • FBX30
  • FBX30FLJ11071
  • FBXO30
  • FBXW6
  • FBXW7
  • hAgo
  • homolog of C elegans sel-10
  • SCF(Fbw7)
  • SEL10
  • SEL-10DKFZp686F23254
  • SEL10FBW6

Background

Auxin-inducible degron (AID) technology allows rapid depletion of proteins in animal cells and fungi. Auxin includes chemicals such as IAA (indole-3-aceticacid) and NAA (1-naphtalene acetic acid) and includes inhibitors in plants. For the degradation, and SCF ubiquitin ligase complex containing the TIR1 protein is activated via the association of auxin with TIR1. Because the core SCF components are conserved in all eukaryotic cells, it is possible to transplant the degradation pathway to yeast and mammalian cells by expressing TIR1. In those cells expressing TIR1, a protein fused with degron (AID degron) derived from AUX/IAA can be rapidly degraded in the presence of auxin. Anti-mini-AID tag can detected both mini-AID-tag and full-length AID-tag.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FBXW7/Cdc4
Antibody (OTI6F5) - A ...
NBP2-71995
FBXW7/Cdc4 Antibody (OTI6F5) - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: WB, IHC

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Bioinformatics

Gene Symbol FBXW7