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FBXW7/Cdc4 Antibody (OTI6F5) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI6F5
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71995AF350
Alexa Fluor 405
NBP2-71995AF405
Alexa Fluor 488
NBP2-71995AF488
Alexa Fluor 532
NBP2-71995AF532
Alexa Fluor 594
NBP2-71995AF594
Alexa Fluor 647
NBP2-71995AF647
Alexa Fluor 700
NBP2-71995AF700
Alexa Fluor 750
NBP2-71995AF750
Allophycocyanin
NBP2-71995APC
Biotin
NBP2-71995B
DyLight 350
NBP2-71995UV
DyLight 405
NBP2-71995V
DyLight 488
NBP2-71995G
DyLight 550
NBP2-71995R
DyLight 594
NBP2-71995DL594
DyLight 650
NBP2-71995C
DyLight 680
NBP2-71995FR
DyLight 755
NBP2-71995IR
FITC
NBP2-71995F
HRP
NBP2-71995H
Janelia Fluor 525
NBP2-71995JF525
Janelia Fluor 549
NBP2-71995JF549
Janelia Fluor 585
NBP2-71995JF585
Janelia Fluor 635
NBP2-71995JF635
Janelia Fluor 646
NBP2-71995JF646
Janelia Fluor 669
NBP2-71995JF669
PE
NBP2-71995PE
PerCP
NBP2-71995PCP
Unconjugated
NBP2-45623
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45623
Azide and BSA Free
NBP2-71995
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FBXW7/Cdc4 Antibody (OTI6F5) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-354 of human FBXW7 (NP_361014) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
FBXW7
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for FBXW7/Cdc4 Antibody (OTI6F5) [CoraFluor™ 1]

  • AGO
  • AGOFBXO30
  • Archipelago Homolog
  • archipelago, Drosophila, homolog of
  • Cdc4
  • CDC4FLJ16457
  • F-box and WD repeat domain containing 7
  • F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila)
  • F-box and WD-40 domain-containing protein 7
  • F-box protein FBW7
  • F-box protein FBX30
  • F-box protein SEL-10
  • F-box/WD repeat-containing protein 7
  • FBW6
  • Fbw7
  • FBW7hCdc4
  • FBX30
  • FBX30FLJ11071
  • FBXO30
  • FBXW6
  • FBXW7
  • hAgo
  • homolog of C elegans sel-10
  • SCF(Fbw7)
  • SEL10
  • SEL-10DKFZp686F23254
  • SEL10FBW6

Background

Auxin-inducible degron (AID) technology allows rapid depletion of proteins in animal cells and fungi. Auxin includes chemicals such as IAA (indole-3-aceticacid) and NAA (1-naphtalene acetic acid) and includes inhibitors in plants. For the degradation, and SCF ubiquitin ligase complex containing the TIR1 protein is activated via the association of auxin with TIR1. Because the core SCF components are conserved in all eukaryotic cells, it is possible to transplant the degradation pathway to yeast and mammalian cells by expressing TIR1. In those cells expressing TIR1, a protein fused with degron (AID degron) derived from AUX/IAA can be rapidly degraded in the presence of auxin. Anti-mini-AID tag can detected both mini-AID-tag and full-length AID-tag.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FBXW7/Cdc4
Antibody (OTI6F5) [Co ...
NBP2-71995CL1
FBXW7/Cdc4 Antibody (OTI6F5) [CoraFluor™ 1]
Species: Hu, Mu, Rt
Applications: WB, IHC

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Bioinformatics

Gene Symbol FBXW7