![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0008.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500.")
| Reactivity | Hu, Mu, Rt, Ch, XpSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Carrier-protein conjugated synthetic peptide surrounding phospho Tyr576 of human FAK. The exact sequence is proprietary. |
| Modification | p Tyr576 |
| Predicted Species | Chicken (100%), Xenopus (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PTK2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Western_Blot_5930.jpg)
![Simple Western AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16332.jpg)
![Intracellular Staining by Flow Cytometry AKT [p Ser473] Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/Akt3_AF887_Flow_Cytometry_8283.jpg)
![Simple Western EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Simple_Western_21454.jpg)
![Western Blot EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Western_Blot_21511.jpg)
![Immunocytochemistry EphB2 Antibody [Unconjugated]](https://images.novusbio.com/images2/EphB2_AF467_Immunocytochemistry__Immunofluorescence_17288.jpg)
![Immunohistochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunohistochemistry_20892.jpg)
![Immunocytochemistry EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Immunocytochemistry__Immunofluorescence_21143.jpg)
![Western Blot EGFR Antibody [Unconjugated]](https://images.novusbio.com/images2/EGF_R_AF231_Western_Blot_19925.jpg)
![FAK [p Tyr576] Antibody](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "FAK [p Tyr576] Antibody")
Secondary Antibodies |
Isotype Controls |
Research Areas for FAK Antibody (NBP3-13071)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PTK2 |
![Immunocytochemistry/Immunofluorescence: FAK [p Tyr576] Antibody [NBP3-13071] - FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein at cell membrane and nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FAK (phospho Tyr576) protein stained by FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13071-img0002.jpg "Immunocytochemistry/Immunofluorescence: FAK [p Tyr576] Antibody [NBP3-13071] - FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein at cell membrane and nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FAK (phospho Tyr576) protein stained by FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein by western blot analysis. Left: Untreated (-) and EGF-treated (+, 50 ng/ml for 30 min) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE. Right: Untreated (-) and EGF-treated (+, 200 ng/ml for 30 min) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE. The membranes were blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:3000.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0001.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein by western blot analysis. Left: Untreated (-) and EGF-treated (+, 50 ng/ml for 30 min) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE. Right: Untreated (-) and EGF-treated (+, 200 ng/ml for 30 min) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE. The membranes were blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:3000.")
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0003.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0004.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0005.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0006.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Untreated (-) and treated (+) HepG2 whole cell extract (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FAK-[p-Tyr576]-Antibody-Western-Blot-NBP3-13071-img0007.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Untreated (-) and treated (+) HepG2 whole cell extract (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13071_rabbit-fak-p-tyr-576-pab-91020242041236.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13071_rabbit-fak-p-tyr-576-pab-9102024205680.jpg "Western Blot: FAK [p Tyr576] Antibody [NBP3-13071] - Untreated (-) and treated (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody (NBP3-13071) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: FAK [p Tyr576] Antibody [NBP3-13071] - FAK antibody detects FAK protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded rat lung.FAK stained by FAK antibody (NBP3-13071) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13071_rabbit-fak-p-tyr-576-pab-910202420115066.jpg "Immunohistochemistry-Paraffin: FAK [p Tyr576] Antibody [NBP3-13071] - FAK antibody detects FAK protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded rat lung.FAK stained by FAK antibody (NBP3-13071) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunocytochemistry Paxillin Antibody [Unconjugated]](https://images.novusbio.com/images2/Paxillin_AF4259_Immunocytochemistry_10249.jpg)
![Western Blot Paxillin Antibody [Unconjugated]](https://images.novusbio.com/images2/Paxillin_AF4259_Western_Blot_5429.jpg)
![Simple Western Paxillin Antibody [Unconjugated]](https://images.novusbio.com/images2/Paxillin_AF4259_Simple_Western_17510.jpg)
![Western Blot Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/Src_AF2685_Western_Blot_5209.jpg)
![Simple Western Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/16327.jpg)
![Immunohistochemistry Src [p Tyr419] Antibody [Unconjugated]](https://images.novusbio.com/images2/Src_AF2685_Immunohistochemistry_7158.jpg)
![Simple Western PYK2/FAK2 [p Tyr402] Antibody (592918) [Unconjugated]](https://images.novusbio.com/images2/PYK2_MAB6210_Simple_Western_16639.jpg)
![Western Blot PYK2/FAK2 [p Tyr402] Antibody (592918) [Unconjugated]](https://images.novusbio.com/images2/PYK2_MAB6210_Western_Blot_9430.jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![Flow Cytometry Integrin beta 1/CD29 Antibody (P5D2) [Unconjugated]](https://images.novusbio.com/images2/Integrin_beta_1_MAB17781_Flow_Cytometry_17077.jpg)
![Immunocytochemistry Integrin beta 1/CD29 Antibody (P5D2) [Unconjugated]](https://images.novusbio.com/images2/Integrin_beta_1_MAB17781_Immunocytochemistry__Immunofluorescence_17398.jpg)
![Flow Cytometry Integrin beta 1/CD29 Antibody (P5D2) [Unconjugated]](https://images.novusbio.com/images2/Integrin_beta_1_MAB17781_Flow_Cytometry_23861.jpg)
![Simple Western Vinculin Antibody (728526) [Unconjugated]](https://images.novusbio.com/images2/Vinculin_MAB6896_Simple_Western_16408.jpg)
![Western Blot Vinculin Antibody (728526) [Unconjugated]](https://images.novusbio.com/images2/Vinculin_MAB6896_Western_Blot_11566.jpg)
![Immunohistochemistry Vinculin Antibody (728526) [Unconjugated]](https://images.novusbio.com/images2/Vinculin_MAB6896_Immunohistochemistry_11621.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")