![Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Western blot analysis of FAK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbi](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Western-Blot-NBP2-67327-img0012.jpg "Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Western blot analysis of FAK on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbi")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | SR46-04 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within human FAK aa 700-740. (SwissProt: Q05397 Human; SwissProt: P34152 Mouse; SwissProt: O35346 Rat) |
| Localization | Cytoplasm, Nucleus, Cell membrane, Cell junction. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | PTK2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for FAK Antibody (NBP2-67327)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PTK2 |
![Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunocytochemistry-Immunofluorescence-NBP2-67327-img0007.jpg "Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0011.jpg "Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m")
![Flow Cytometry: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of Hela cells with FAK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Flow-Cytometry-NBP2-67327-img0001.jpg "Flow Cytometry: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of Hela cells with FAK antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of FAK on different lysates using anti-FAK antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse spleen](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Western-Blot-NBP2-67327-img0006.jpg "Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of FAK on different lysates using anti-FAK antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse spleen")
![Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunocytochemistry-Immunofluorescence-NBP2-67327-img0002.jpg "Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded human spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0003.jpg "Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded human spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded mouse brain tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0004.jpg "Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded mouse brain tissue using anti-FAK antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0005.jpg "Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded mouse spleen tissue using anti-FAK antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded huamn liver cancer tissue using anti-FAK antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0008.jpg "Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded huamn liver cancer tissue using anti-FAK antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0009.jpg "Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m")
![Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m](http://images.novusbio.com/fullsize/FAK-Antibody-SR46-04-Immunohistochemistry-Paraffin-NBP2-67327-img0010.jpg "Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-FAK antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")