Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP5/E-FABP Antigen Affinity-purified Polyclonal ...read more
FABP5/E-FABP was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) at 10 µg/mL for 3 hours at ...read more
Simple Western lane view shows lysates of human heart tissue and human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP5/E‑FABP at approximately 21 kDa (as indicated) using 10 µg/mL ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FABP5/E‑FABP at ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FABP5/E-FABP knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat ...read more
E. coli-derived recombinant human FABP5/E‑FABP Ala2-Glu135 Accession # Q01469
Specificity
Detects human FABP5/E‑FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse (rm) FABP5 is observed and less than 5% cross-reactivity with recombinant human FABP1, -2, -3, -4, -6, -7, and rmFABP9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FABP5
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for FABP5/E-FABP Antibody [Unconjugated]
EFABP
E-FABP
E-FABPPAFABP
FABP5
fatty acid binding protein 5 (psoriasis-associated)
Fatty acid-binding protein 5
PA-FABPepidermal
Psoriasis-associated fatty acid-binding protein homolog
Background
Human FABP-5, also known as epidermal fatty acid binding protein (E-FABP), is a 15 kDa member of a cytosolic fatty acid binding protein superfamily. It is associated with keratinocytes and adipocytes and is suggested to promote fatty acid availability to enzymes, protect cell structures from fatty acid attack, and target fatty acids to nuclear transcription factors. The amino acid sequence of human FABP5 is 80%, 81% and 92% identical to that of mouse, rat and bovine FABP5, respectively.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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