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FABP5/E-FABP Antibody [Unconjugated]

Images

 
Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FABP5/E-FABP Antigen Affinity-purified Polyclonal ...read more
FABP5/E-FABP was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human FABP5/E-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3077) at 10 µg/mL for 3 hours at ...read more
Simple Western lane view shows lysates of human heart tissue and human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP5/E‑FABP at approximately 21 kDa (as indicated) using 10 µg/mL ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FABP5/E‑FABP at ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FABP5/E-FABP knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC/IF, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

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FABP5/E-FABP Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human FABP5/E‑FABP
Ala2-Glu135
Accession # Q01469
Specificity
Detects human FABP5/E‑FABP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse (rm) FABP5 is observed and less than 5% cross-reactivity with recombinant human FABP1, -2, -3, -4, -6, -7, and rmFABP9 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FABP5
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Knockout Validated
  • Simple Western 10-12.5 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3077 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FABP5/E-FABP Antibody [Unconjugated]

  • EFABP
  • E-FABP
  • E-FABPPAFABP
  • FABP5
  • fatty acid binding protein 5 (psoriasis-associated)
  • Fatty acid-binding protein 5
  • PA-FABPepidermal
  • Psoriasis-associated fatty acid-binding protein homolog

Background

Human FABP-5, also known as epidermal fatty acid binding protein (E-FABP), is a 15 kDa member of a cytosolic fatty acid binding protein superfamily. It is associated with keratinocytes and adipocytes and is suggested to promote fatty acid availability to enzymes, protect cell structures from fatty acid attack, and target fatty acids to nuclear transcription factors. The amino acid sequence of human FABP5 is 80%, 81% and 92% identical to that of mouse, rat and bovine FABP5, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FABP5/E-FABP Antibody (AF3077)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Macaca fuscata (Japanese Macaque).

We have publications tested in 4 applications: ICC, IHC-Fr, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(2)
IHC-Fr
(1)
Immunoprecipitation
(1)
Western Blot
(7)
All Applications
Filter By Species
Human
(4)
Mouse
(2)
Primate - Macaca fuscata (Japanese Macaque)
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF3077 Applications Species
A Cheng, W Jia, I Kawahata, K Fukunaga A novel fatty acid-binding protein 5 and 7 inhibitor ameliorates oligodendrocyte injury in multiple sclerosis mouse models EBioMedicine, 2021-10-05;72(0):103582. 2021-10-05 [PMID: 34624687] (Western Blot, Mouse) Western Blot Mouse
Q Guo, I Kawahata, T Degawa, Y Ikeda-Mats, M Sun, F Han, K Fukunaga Fatty Acid-Binding Proteins Aggravate Cerebral Ischemia-Reperfusion Injury in Mice Biomedicines, 2021-05-10;9(5):. 2021-05-10 [PMID: 34068550] (Western Blot, Mouse) Western Blot Mouse
Y Wang, Y Shinoda, A Cheng, I Kawahata, K Fukunaga Epidermal Fatty Acid-Binding Protein 5 (FABP5) Involvement in Alpha-Synuclein-Induced Mitochondrial Injury under Oxidative Stress Biomedicines, 2021-01-22;9(2):. 2021-01-22 [PMID: 33499263] (ICC, Western Blot, Human) ICC, Western Blot Human
A Cheng, I Kawahata, K Fukunaga Fatty Acid Binding Protein 5 Mediates Cell Death by Psychosine Exposure through Mitochondrial Macropores Formation in Oligodendrocytes Biomedicines, 2020-12-20;8(12):. 2020-12-20 [PMID: 33419250] (ICC, Immunoprecipitation, Western Blot, Human) ICC, Immunoprecipitation, Western Blot Human
Chen P, Zhao D, Li J et al. Symbiotic Macrophage-Glioma Cell Interactions Reveal Synthetic Lethality in PTEN-Null Glioma Cancer Cell 6/10/2019 [PMID: 31185211]
Liraz Levi, Zeneng Wang, Mary Kathryn Doud, Stanley L. Hazen, Noa Noy Saturated fatty acids regulate retinoic acid signalling and suppress tumorigenesis by targeting fatty acid-binding protein 5 Nature Communications 11/23/2015 [PMID: 26592976]
William R. Swindell, Henriette A. Remmer, Mrinal K. Sarkar, Xianying Xing, Drew H. Barnes, Liza Wolterink et al. Proteogenomic analysis of psoriasis reveals discordant and concordant changes in mRNA and protein abundance Genome Medicine 12/1/2015 [PMID: 26251673]
Thulasiraman P, McAndrews D, Mohiudddin I Curcumin restores sensitivity to retinoic acid in triple negative breast cancer cells. BMC Cancer, 2014-09-27;14(0):724. 2014-09-27 [PMID: 25260874] (Western Blot, Human) Western Blot Human
Liraz Levi, Glenn Lobo, Mary Kathryn Doud, Johannes von Lintig, Darcie Seachrist, Gregory P. Tochtrop et al. Genetic Ablation of the Fatty Acid–Binding Protein FABP5 Suppresses HER2-Induced Mammary Tumorigenesis Cancer Research 8/1/2013 [PMID: 23722546]
Kannan-Thulasiraman P, Seachrist DD, Mahabeleshwar GH, Jain MK, Noy N Fatty acid-binding protein 5 and PPARbeta/delta are critical mediators of epidermal growth factor receptor-induced carcinoma cell growth. J. Biol. Chem., 2010-04-27;285(25):19106-15. 2010-04-27 [PMID: 20424164] (Western Blot, Human) Western Blot Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol FABP5
Uniprot