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FABP2/I-FABP Antibody

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Western Blot: FABP2/I-FABP Antibody [NB100-59746] - (0.001ug/ml) staining of Human Duodenum (A) and (0.003ug/ml) Ileum (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence
Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody [NB100-59746] - (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: FABP2/I-FABP Antibody [NB100-59746] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor ...read more

Product Details

Summary
Reactivity Hu, Mu, Fi, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FABP2/I-FABP Antibody Summary

Immunogen
Peptide with sequence C-EGVEAKRIFKKD corresponding to C-Terminus according to NP_000125.2.
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FABP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10ug/ml
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2 - 3 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.001- 0.003 ug/ml
Application Notes
Western blot: Approx 15-16kDa band observed in Human Ileum and Duodenum lysates (calculated MW of 15.2kDa according to NP_000125.1). Recommended concentration: 0.001-0.003ug/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Human Small Intestine shows strong staining of basement membrane and weaker staining of enterocytes. Recommended concentration: 2-3ug/ml.
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Duodenum Whole Tissue Lysate (Adult Whole Normal)
FABP2/I-FABP Overexpression Lysate
Control Peptide
FABP2/I-FABP Protein (NB100-59746PEP)
Publications
Read Publications using
NB100-59746 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22790967). Fish reactivity reported in multiple pieces of scientific literature. Predicted cross-reactivity based on sequence identity: Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FABP2/I-FABP Antibody

  • FABP2
  • FABPI
  • fatty acid binding protein 2, intestinal
  • Fatty acid-binding protein 2
  • fatty acid-binding protein, intestinal
  • IFABP
  • I-FABP
  • I-FABPMGC133132
  • Intestinal FABP
  • Intestinal-type fatty acid-binding protein

Background

The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FABP2/I-FABP Antibody (NB100-59746)(8)

We have publications tested in 2 confirmed species: Mouse, Fish.

We have publications tested in 3 applications: IF/IHC, Immunohistochemistry, WB.


Filter By Application
IF/IHC
(2)
Immunohistochemistry
(1)
WB
(2)
All Applications
Filter By Species
Mouse
(2)
Fish
(3)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-59746 Applications Species
Verdile N, Pasquariello R, Brevini T, Gandolfi F The 3D Pattern of the Rainbow Trout (Oncorhynchus mykiss) Enterocytes and Intestinal Stem Cells IJMS 2020-12-02 [PMID: 33276531]
Yagishita Y, McCallum ML, Kensler TW, Wakabayashi N Constitutive Activation of Nrf2 in Mice Expands Enterogenesis in Small Intestine Through Negative Regulation of Math1 Cell Mol Gastroenterol Hepatol 2020-09-05 [PMID: 32896624] (Mouse) Mouse
Verdile N, Cardinaletti, G Faccenda F et al. Ectopic stem cell niches sustain rainbow trout (Oncorhynchus mykiss) intestine absorptive capacity when challenged with a plant protein-rich diet Aquaculture 2023-02-01 (Immunohistochemistry, Fish) Immunohistochemistry Fish
Estensoro I, Ballester-Lozano G, Benedito-Palos L et al. Dietary Butyrate Helps to Restore the Intestinal Status of a Marine Teleost (Sparus aurata) Fed Extreme Diets Low in Fish Meal and Fish Oil. PLoS ONE. 2016-11-29 [PMID: 27898676] (IF/IHC, Fish) IF/IHC Fish
Krogdahl A, Gajardo K, Kortner TM et al. Soya Saponins Induce Enteritis in Atlantic Salmon (Salmo salar L.). J Agric Food Chem 2015-04-22 [PMID: 25798699]
Venold FF, Penn MH, Thorsen J et al. Intestinal fatty acid binding protein (fabp2) in Atlantic salmon (Salmo salar): Localization and alteration of expression during development of diet induced enteritis Comp Biochem Physiol A Mol Integr Physiol 2012-09-20 [PMID: 23000355] (WB, IF/IHC, Fish) WB, IF/IHC Fish
Cheng J, Krausz KW, Tanaka N, Gonzalez FJ. Chronic exposure to rifaximin causes hepatic steatosis in pregnane x receptor-humanized mice Toxicol Sci 2012-10-01 [PMID: 22790967] (WB, Mouse) WB Mouse
Takakura Y, Yoshioka K, Umekawa T et al. Thr54 allele of the FABP2 gene affects resting metabolic rate and visceral obesity. Diabetes Res Clin Pract 2005-01-01 [PMID: 15620432]

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Bioinformatics

Gene Symbol FABP2
Entrez
Uniprot