Western Blot: FABP2/I-FABP Antibody [NB100-59746] - (0.001ug/ml) staining of Human Duodenum (A) and (0.003ug/ml) Ileum (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence
Immunohistochemistry-Paraffin: FABP2/I-FABP Antibody [NB100-59746] - (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: FABP2/I-FABP Antibody [NB100-59746] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor ...read more
Western blot: Approx 15-16kDa band observed in Human Ileum and Duodenum lysates (calculated MW of 15.2kDa according to NP_000125.1). Recommended concentration: 0.001-0.003ug/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Human Small Intestine shows strong staining of basement membrane and weaker staining of enterocytes. Recommended concentration: 2-3ug/ml.
Theoretical MW
15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Mouse reactivity reported in scientific literature (PMID: 22790967). Fish reactivity reported in multiple pieces of scientific literature. Predicted cross-reactivity based on sequence identity: Rat, Canine, Porcine, Bovine.
Packaging, Storage & Formulations
Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified
Alternate Names for FABP2/I-FABP Antibody
FABP2
FABPI
fatty acid binding protein 2, intestinal
Fatty acid-binding protein 2
fatty acid-binding protein, intestinal
IFABP
I-FABP
I-FABPMGC133132
Intestinal FABP
Intestinal-type fatty acid-binding protein
Background
The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Verdile N, Cardinaletti, G Faccenda F et al. Ectopic stem cell niches sustain rainbow trout (Oncorhynchus mykiss) intestine absorptive capacity when challenged with a plant protein-rich diet Aquaculture 2023-02-01 (Immunohistochemistry, Fish)
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