![Immunohistochemistry-Paraffin: Apolipoprotein CIII Antibody [NB600-610] - Tissue: liver.](http://images.novusbio.com/fullsize/Apolipoprotein-CIII-Antibody-Immunohistochemistry-Paraffin-NB600-610-img0001.jpg "Immunohistochemistry-Paraffin: Apolipoprotein CIII Antibody [NB600-610] - Tissue: liver.")
| Reactivity | Hu, BvSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Description | This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. |
| Immunogen | produced from Apolipoprotein CIIII derived from human plasma (Uniprot: P02656) |
| Specificity | Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein C-III and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | APOC3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This product have been tested in immunohistochemistry and used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, and for western blotting for highly sensitive qualitative analysis. When performing immunoblotting assays - do not block with blotto. Blotto formulations tend to contain apoLipoprotein Type C-III and will neutralize antibody activity. |
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| Storage | Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles. |
| Buffer | 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 |
| Preservative | 0.01% Sodium Azide |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
ELISA | Human | 11/03/2009 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Apolipoprotein CIII Antibody (NB600-610)Find related products by research area.
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