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Ezrin Antibody (CL2375)

Images

 
Western Blot: Ezrin Antibody (CL2375) [NBP2-36759] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (CL2375) [NBP2-36759] - Staining of A-431 cells using the Anti-EZR monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and ...read more
Immunohistochemistry: Ezrin Antibody (CL2375) [NBP2-36759] - Staining of human skeletal muscle shows absence of immunoreactivity.
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (CL2375) [NBP2-36759] - Staining of U-251 cells using the Anti-EZR monoclonal antibody, showing specific staining in the plasma membrane in green. Microtubule- and ...read more
Immunohistochemistry: Ezrin Antibody (CL2375) [NBP2-36759] - Staining of human small intestine shows strong membranous combined with moderate cytoplasmic immunoreactivity in glandular epithelium cells.
Immunohistochemistry: Ezrin Antibody (CL2375) [NBP2-36759] - Staining of human colon shows strong membranous immunoreactivity in glandular epithelium and lamina propria cells.
Immunohistochemistry: Ezrin Antibody (CL2375) [NBP2-36759] - Staining of human placenta shows strong membranous positivity in the trophoblast.
Immunohistochemistry: Ezrin Antibody (CL2375) [NBP2-36759] - Staining of human stomach shows strong membranous combined with moderate cytoplasmic immunoreactivity in glandular epithelium and lamina propria cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL2375
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Ezrin Antibody (CL2375) Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:REEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLE
Epitope
KAERELSEQIQRALQ
Predicted Species
Mouse (93%), Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EZR
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000-1:10000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Control Peptide
Ezrin Recombinant Protein Antigen (NBP2-36759PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Ezrin Antibody (CL2375)

  • CVIL
  • CVL
  • Cytovillin 2
  • Cytovillin
  • DKFZp762H157
  • EZR
  • Ezrin
  • FLJ26216
  • p81
  • VIL2
  • VIL2MGC1584
  • villin 2 (ezrin)
  • Villin 2
  • villin-2

Background

Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. It is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity and is expressed in epithelial cells but not in mesenchymal cells. It is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ezrin Antibody (NBP2-36759) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ezrin Antibody (NBP2-36759) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EZR
Uniprot