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Ezrin Antibody (6F1A9) - BSA Free

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Western Blot: Ezrin Antibody (6F1A9) [NBP2-52465] - Analysis using EZR mouse mAb against MCF-7 (1), Hela (2), A431 (3), Hek293 (4), SK-N-SH (5), Jurkat (6), HepG2 (7), NIH/3T3 (8), and Cos7 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody (6F1A9) [NBP2-52465] - Analysis of A549 cells using EZR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Flow Cytometry: Ezrin Antibody (6F1A9) [NBP2-52465] - Analysis of MCF-7 cells using EZR mouse mAb (green) and negative control (red).
Western Blot: Ezrin Antibody (6F1A9) [NBP2-52465] - Analysis using EZR mAb against HEK293 (1) and EZR (AA: 292-464)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: Ezrin Antibody (6F1A9) [NBP2-52465] - Analysis using EZR mAb against human EZR (AA: 292-464) recombinant protein. (Expected MW is 47.1 kDa)
Flow Cytometry: Ezrin Antibody (6F1A9) [NBP2-52465] - Analysis of Hela cells using EZR mouse mAb (green) and negative control (red).
ELISA: Ezrin Antibody (6F1A9) [NBP2-52465] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, CyTOF-ready
Clone
6F1A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Catalog# & Formulation Size Price
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Ezrin Antibody (6F1A9) - BSA Free Summary

Immunogen
Purified recombinant fragment of human Ezrin (AA: 292-464) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EZR
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:100-1:400
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
69.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Ezrin Antibody (6F1A9) - BSA Free

  • CVIL
  • CVL
  • Cytovillin 2
  • Cytovillin
  • DKFZp762H157
  • EZR
  • Ezrin
  • FLJ26216
  • p81
  • VIL2
  • VIL2MGC1584
  • villin 2 (ezrin)
  • Villin 2
  • villin-2

Background

Ezrin serves as a tyrosine kinase substrate and is phosphorylated in EGF-stimulated cells. It is a cytoplasmic protein enriched in microvilli and other cell surfaces. Ezrin has an actin-binding capacity and is expressed in epithelial cells but not in mesenchymal cells. It is also expressed by certain epithelial tumors, such as renal cell adenocarcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ezrin Antibody (NBP2-52465) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EZR