![Western Blot: ETV5/ERM Antibody [NBP2-14950] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ETV5-ERM-Antibody-Western-Blot-NBP2-14950-img0013.jpg "Western Blot: ETV5/ERM Antibody [NBP2-14950] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Po, BvSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ETV5/ERM. The exact sequence is proprietary. |
| Predicted Species | Rat (95%), Porcine (98%), Bovine (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ETV5 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-Fr Assay dependent |
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| Theoretical MW | 58 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Western Blot Ets-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Ets-1_AF7284_Western_Blot_11854.jpg)
![Immunohistochemistry Ets-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Ets1_AF7284_Immunohistochemistry_22698.jpg)
| Publication using NBP2-14950 | Applications | Species |
|---|---|---|
| Pan Y, Duron C, Bush EC et al. Graph complexity analysis identifies an ETV5 tumor-specific network in human and murine low-grade glioma PLoS ONE 2018-05-22 [PMID: 29787563] (IF/IHC, Mouse) | IF/IHC | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for ETV5/ERM Antibody (NBP2-14950)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: ETV5/ERM Antibody [NBP2-14950] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERM protein stained by ERM antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um](http://images.novusbio.com/fullsize/ETV5-ERM-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-14950-img0008.jpg "Immunocytochemistry/Immunofluorescence: ETV5/ERM Antibody [NBP2-14950] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERM protein stained by ERM antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 um")
![Western Blot: ETV5/ERM Antibody [NBP2-14950] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ETV5-ERM-Antibody-Western-Blot-NBP2-14950-img0011.jpg "Western Blot: ETV5/ERM Antibody [NBP2-14950] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: ETV5/ERM Antibody [NBP2-14950] - PC14 xenograft, using ERM antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ERM-Etv5-Antibody-Immunohistochemistry-Paraffin-NBP2-14950-img0002.jpg "Immunohistochemistry-Paraffin: ETV5/ERM Antibody [NBP2-14950] - PC14 xenograft, using ERM antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: ETV5/ERM Antibody [NBP2-14950] - ETV5/ERM antibody detects ETV5/ERM protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. ETV5/ERM antibody (NBP2-14950) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-14950_rabbit-polyclonal-etv5-erm-antibody-91020242091452.jpg "Immunohistochemistry-Paraffin: ETV5/ERM Antibody [NBP2-14950] - ETV5/ERM antibody detects ETV5/ERM protein at nucleus in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. ETV5/ERM antibody (NBP2-14950) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")