![Western Blot: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Escherichia coli expressed recombinant human EPO protein were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (NBP3-25455) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-2120249371541.jpg "Western Blot: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Escherichia coli expressed recombinant human EPO protein were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (NBP3-25455) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
| Reactivity | Hu, Ca, FeSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clone | HL1794 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein of human Erythropoietin/EPO |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | EPO |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 21 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
![Simple Western STAT5b Antibody [Unconjugated]](https://images.novusbio.com/images2/16321.jpg)
![Knockout Validated STAT5b Antibody [Unconjugated]](https://images.novusbio.com/images2/STAT5b_AF1584_Knockout_Validated_21968.jpg)
![Immunocytochemistry STAT5b Antibody [Unconjugated]](https://images.novusbio.com/images2/STAT5b_AF1584_Immunocytochemistry__Immunofluorescence_17603.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for Erythropoietin/EPO Antibody (NBP3-25455)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | EPO |
![ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Indirect ELISA analysis was performed by coating the plate with recombinant CHO cells expressed, full-length human EPO protein (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (NBP3-25455) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-21202493913.jpg "ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Indirect ELISA analysis was performed by coating the plate with recombinant CHO cells expressed, full-length human EPO protein (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (NBP3-25455) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.")
![Western Blot: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (NBP3-25455) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-2120249432813.jpg "Western Blot: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - HepG2 whole cell extract and conditioned medium (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (NBP3-25455) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Recombinant human EPO protein (CHO cells expressed) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (NBP3-25455) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-212024941491.jpg "Western Blot: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Recombinant human EPO protein (CHO cells expressed) was separated by 12% SDS-PAGE, and the membrane was blotted with EPO antibody [HL1794] (NBP3-25455) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis. Sample: Paraffin-embedded dog kidney. EPO stained by EPO antibody [HL1794] (NBP3-25455) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-212024941438.jpg "Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis. Sample: Paraffin-embedded dog kidney. EPO stained by EPO antibody [HL1794] (NBP3-25455) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. EPO stained by EPO antibody [HL1794] (NBP3-25455) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-212024941443.jpg "Immunohistochemistry-Paraffin: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - EPO antibody [HL1794] detects secreted EPO protein by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. EPO stained by EPO antibody [HL1794] (NBP3-25455) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below. Capture: EPO antibody [HL1794] (NBP3-25455) (5 ug/mL) Detection: HRP-conjugated EPO antibody [HL1793] (NBP3-25454) (1 ug/mL) Please notice that NBP3-25454 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25455. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-2120249355565.jpg "ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below. Capture: EPO antibody [HL1794] (NBP3-25455) (5 ug/mL) Detection: HRP-conjugated EPO antibody [HL1793] (NBP3-25454) (1 ug/mL) Please notice that NBP3-25454 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25455. Please contact us for custom HRP-conjugated antibody.")
![ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human EPO protein, His and GST tag (active) (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (NBP3-25455) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-2120249432861.jpg "ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human EPO protein, His and GST tag (active) (500-7.81 ng/mL). Coated protein was probed with EPO antibody [HL1794] (NBP3-25455) (1 ug/mL). Goat anti-rabbit IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.")
![ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below. Capture: EPO antibody [HL1793] (NBP3-25454) (5 ug/mL) Detection: HRP-conjugated EPO antibody [HL1794] (NBP3-25455) (1 ug/mL) Please notice that NBP3-25455 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25454. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25455_rabbit-erythropoietin-epo-mab-hl1794-azide-and-bsa-free-212024941445.jpg "ELISA: Erythropoietin/EPO Antibody (HL1794) - Azide and BSA Free [NBP3-25455] - Sandwich ELISA detection of recombinant CHO cells expressed, full-length human EPO protein using antibodies as below. Capture: EPO antibody [HL1793] (NBP3-25454) (5 ug/mL) Detection: HRP-conjugated EPO antibody [HL1794] (NBP3-25455) (1 ug/mL) Please notice that NBP3-25455 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25454. Please contact us for custom HRP-conjugated antibody.")
![SDS-PAGE IL-3 [Unconjugated]](https://images.novusbio.com/images2/IL-3_203-IL_345.jpg)
![Bioactivity IL-3 [Unconjugated]](https://images.novusbio.com/images2/IL-3_203-IL_347.jpg)
![SEC-MALS IL-3 [Unconjugated]](https://images.novusbio.com/images/203-il_recombinant-human-il-3-protein-sec-mals-133202482656..jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Bioactivity GM-CSF [Unconjugated]](https://images.novusbio.com/images/protein/7954-gmcf_recombinant-human-gm-csf-cho-expressed-protein-cf-bioactivity-272020121514.jpg)
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![Neutralization G-CSF Antibody (67604) [Unconjugated]](https://images.novusbio.com/images2/G-CSF_MAB414_Block_Neutralize_9188.jpg)
![Bioactivity Thrombopoietin/THPO [Unconjugated]](https://images.novusbio.com/images/protein/288-tpncf_recombinant-human-thrombopoietin-ns0-expressed-protein-cf-bioactivity-272020135047.jpg)
![Western Blot STAT5a/b Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/STAT5b_AF2168_Western_Blot_5176.jpg)
![Simple Western STAT5a/b Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/16339.jpg)
![Chromatin Immunoprecipitation (ChIP) STAT5a/b Antibody [Unconjugated] - Pan Specific](https://images.novusbio.com/images2/STAT5b_AF2168_Chromatin_Immunoprecipitation_11246.jpg)
![Immunohistochemistry CD34 Antibody [Unconjugated]](https://images.novusbio.com/images2/CD34_AF4117_Immunohistochemistry_6824.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")