![Western Blot: ERp57/PDIA3 Antibody [NBP2-16369] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with ERp57 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Western-Blot-NBP2-16369-img0008.jpg "Western Blot: ERp57/PDIA3 Antibody [NBP2-16369] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with ERp57 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Pm, Rb, RM, ShSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ERp57. The exact sequence is proprietary. |
| Localization | Endoplasmic reticulum lumen (By similarity); Melanosome |
| Marker | Endoplasmic Reticulum Marker |
| Predicted Species | Porcine (94%), Chimpanzee (100%), Rabbit (94%), Bovine (93%), Rhesus Macaque (98%), Sheep (91%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PDIA3 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 57 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.01% Thimerosal |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for ERp57/PDIA3 Antibody (NBP2-16369)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody [NBP2-16369] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERp57 protein stained by ERp57 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] ( diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16369-img0004.jpg "Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody [NBP2-16369] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ERp57 protein stained by ERp57 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] ( diluted at 1:1000.Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP2-16369] - Mouse testis. ERp57 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP2-16369-img0006.jpg "Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP2-16369] - Mouse testis. ERp57 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: ERp57 Antibody [NBP2-16369] - Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/ERp57-Antibody-Western-Blot-NBP2-16369-img0001.jpg "Western Blot: ERp57 Antibody [NBP2-16369] - Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 7. 5% SDS PAGE gel, diluted at 1:10000.")
![Western Blot: ERp57 Antibody [NBP2-16369] - Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ERp57-Antibody-Western-Blot-NBP2-16369-img0002.jpg "Western Blot: ERp57 Antibody [NBP2-16369] - Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: ERp57/PDIA3 Antibody [NBP2-16369] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Western-Blot-NBP2-16369-img0005.jpg "Western Blot: ERp57/PDIA3 Antibody [NBP2-16369] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ERp57 antibody diluted at 1:500.")
![Western Blot: ERp57/PDIA3 Antibody [NBP2-16369] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERp57 antibody diluted by 1:10000.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Western-Blot-NBP2-16369-img0007.jpg "Western Blot: ERp57/PDIA3 Antibody [NBP2-16369] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ERp57 antibody diluted by 1:10000.")
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP2-16369] - U87 xenograft, using ERp57 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ERp57-Antibody-Immunohistochemistry-Paraffin-NBP2-16369-img0003.jpg "Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP2-16369] - U87 xenograft, using ERp57 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody [Unconjugated]](https://images.novusbio.com/images2/Endorepellin_AF2364_Immunohistochemistry_6744.jpg)
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![Immunocytochemistry BMP-3b/GDF-10 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af1543_human-bmp-3b-gdf-10-affinity-purified-polyclonal-ab-immunocytochemistry-133202414504..jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")