![Western Blot: ERP44 Antibody [NB100-93457] - Staining of K562 lysate with antibody at 0.2 ug/mL (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ERP44-Antibody-Western-Blot-NB100-93457-img0001.jpg "Western Blot: ERP44 Antibody [NB100-93457] - Staining of K562 lysate with antibody at 0.2 ug/mL (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-EQKDSDNYRVFER corresponding to internal region according to NP_055866.1. |
| Epitope | C-EQKDSDNYRVFER |
| Predicted Species | Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | ERP44 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 47 kDa band observed in K562 lysates (calculated MW of 47.0 kDa band according to NP_055866.1). An additional band of unknown identity was also consistently observed at 22 kDa. This band was successfully blocked by incubation with the immunizing peptide. |
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| Theoretical MW | 47 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-93457 | Applications | Species |
|---|---|---|
| Anelli T, Ceppi S, Bergamelli L et al. Sequential steps and checkpoints in the early exocytic compartment during secretory IgM biogenesis. EMBO J 2007-10-03 [PMID: 17805346] |
Secondary Antibodies |
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