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ERK1 Antibody (1A2) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
1A2
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-74032AF350
Alexa Fluor 405
NBP1-74032AF405
Alexa Fluor 488
NBP1-74032AF488
Alexa Fluor 532
NBP1-74032AF532
Alexa Fluor 594
NBP1-74032AF594
Alexa Fluor 647
NBP1-74032AF647
Alexa Fluor 700
NBP1-74032AF700
Alexa Fluor 750
NBP1-74032AF750
Biotin
NBP1-74032B
CoraFluor 1
NBP1-74032CL1
DyLight 350
NBP1-74032UV
DyLight 405
NBP1-74032V
DyLight 488
NBP1-74032G
DyLight 550
NBP1-74032R
DyLight 594
NBP1-74032DL594
DyLight 650
NBP1-74032C
DyLight 680
NBP1-74032FR
DyLight 755
NBP1-74032IR
FITC
NBP1-74032F
HRP
NBP1-74032H
Janelia Fluor 525
NBP1-74032JF525
Janelia Fluor 549
NBP1-74032JF549
Janelia Fluor 669
NBP1-74032JF669
Unconjugated
NBP1-74032-100ul
mFluor Violet 450 SE
NBP1-74032MFV450
mFluor Violet 500 SE
NBP1-74032MFV500
mFluor Violet 610 SE
NBP1-74032MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-74032-100ul
BSA Free
NBP1-74032-50ul
BSA Free
NBP1-74032-100ul
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ERK1 Antibody (1A2) [Janelia Fluor® 646] Summary

Immunogen
Recombinant human ERK1 protein (1-137aa) purified from E. coli
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MAPK3
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for ERK1 Antibody (1A2) [Janelia Fluor® 646]

  • EC 2.7.11
  • ERK1
  • ERK-1
  • ERK1p44-MAPK
  • ERT2
  • Extracellular signal-regulated kinase 1
  • extracellular signal-related kinase 1
  • HS44KDAP
  • HUMKER1A
  • Insulin-stimulated MAP2 kinase
  • MAP kinase 1
  • MAP kinase 3
  • MAP kinase isoform p44
  • MAPK 1
  • MAPK 3
  • MAPK3
  • MGC20180
  • Microtubule-associated protein 2 kinase
  • Mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 3
  • P44ERK1
  • p44-ERK1
  • p44mapk
  • PRKM3
  • PRKM3EC 2.7.11.24

Background

p44 MAP Kinase (Erk1) is a member of a family of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth and differentiation (1,2). Erk1 regulates meiosis and mitosis by phosphorylating a number of transcription factors such as ELK-1. Activation of Erk1 occurs through phosphorylation of threonine 202 and tyrosine 204 (of human MAP kinase) (3). Erk1 is also known to autophosphorylate on tyrosine (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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The role of c-Fos in the regulation of the JC virus gene transcription
c-Fos is a member of the AP-1 transcription factor family under the Fos protein family umbrella, alongside Fra-1, Fra-2 and Fos-B.  Also in the AP-1 transcription family are the Jun proteins, c-Jun, Jun-B and Jun-D.  Each member of the AP-1 transcri...  Read full blog post.
The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.
MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.
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Bioinformatics

Gene Symbol MAPK3