![Immunohistochemistry-Paraffin: ERG Antibody (rERG/6843) [NBP3-13875] - Formalin-fixed, paraffin-embedded human prostate tissue stained with ERG antibody (rERG/6843) at 2ug/ml. HIER: Tris/EDTA pH 9; 95C/45min. Ab: HRP-Polymer:30mins; DAB: 5min.](http://images.novusbio.com/fullsize/ERG-Antibody-rERG-6843-Immunohistochemistry-Paraffin-NBP3-13875-img0001.jpg "Immunohistochemistry-Paraffin: ERG Antibody (rERG/6843) [NBP3-13875] - Formalin-fixed, paraffin-embedded human prostate tissue stained with ERG antibody (rERG/6843) at 2ug/ml. HIER: Tris/EDTA pH 9; 95C/45min. Ab: HRP-Polymer:30mins; DAB: 5min.")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | rERG/6843 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14204) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment (around aa450 to 479) (C-terminus) of human ERG (exact sequence is proprietary) (Uniprot: P11308) |
| Localization | Nucleus |
| Marker | Vascular Endothelial & Prostate Marker |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ERG |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes |
| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for ERG Antibody (NBP3-13875)Find related products by research area.
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![Immunohistochemistry-Paraffin: ERG Antibody (rERG/6843) [NBP3-13875] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using ERG antibody (rERG/6843) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/ERG-Antibody-rERG-6843-Immunohistochemistry-Paraffin-NBP3-13875-img0002.jpg "Immunohistochemistry-Paraffin: ERG Antibody (rERG/6843) [NBP3-13875] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using ERG antibody (rERG/6843) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.")
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