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ERCC8 Antibody

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Western Blot: ERCC8 Antibody [H00001161-B01P] - Analysis of ERCC8 expression in NIH/3T3.
Western Blot: ERCC8 Antibody [H00001161-B01P] - Analysis of ERCC8 expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: ERCC8 Antibody [H00001161-B01P] - Analysis of purified antibody to ERCC8 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: ERCC8 Antibody [H00001161-B01P] - Analysis of ERCC8 expression in transfected 293T cell line by ERCC8 polyclonal antibody. Lane 1: ERCC8 transfected lysate(43.56 KDa). Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

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ERCC8 Antibody Summary

Description
Quality control test: Antibody reactive against mammalian transfected lysate.
Immunogen
ERCC8 (NP_000073.1, 1 a.a. - 396 a.a.) full-length human protein. MLGFLSARQTGLEDPLRLRRAESTRRVLGLELNKDRDVERIHGGGINTLDIEPVEGRYMLSGGSDGVIVLYDLENSSRQSYYTCKAVCSIGRDHPDVHRYSVETVQWYPHDTGMFTSSSFDKTLKVWDTNTLQTADVFNFEETVYSHHMSPVSTKHCLVAVGTRGPKVQLCDLKSGSCSHILQGHRQEILAVSWSPRYDYILATASADSRVKLWDVRRASGCLITLDQHNGKKSQAVESANTAHNGKVNGLCFTSDGLHLLTVGTDNRMRLWNSSNGENTLVNYGKVCNNSKKGLKFTVSCGCSSEFVFVPYGSTIAVYTVYSGEQITMLKGHYKTVDCCVFQSNFQELYSGSRDCNILAWVPSLYEPVPDDDETTTKSQLNPAFEDAWSSSDEEG
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
ERCC8
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
It has been used for WB, Functional and IF.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ERCC8 Antibody

  • CKN1DNA excision repair protein ERCC-8
  • Cockayne syndrome 1 (classical)
  • Cockayne syndrome WD repeat protein CSA
  • CSACockayne syndrome WD-repeat protein CSA
  • excision repair cross-complementing rodent repair deficiency, complementationgroup 8

Background

ERCC8 encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ERCC8 Antibody (H00001161-B01P) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ERCC8
Uniprot