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ERCC8 Antibody (OTI2G1)

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Western Blot: ERCC8 Antibody (2G1) [NBP2-45519] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC8.
Western Blot: ERCC8 Antibody (2G1) [NBP2-45519] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC8.
Immunohistochemistry: ERCC8 Antibody (2G1) [NBP2-45519] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: ERCC8 Antibody (2G1) [NBP2-45519] - Analysis of Carcinoma of Human thyroid tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: ERCC8 Antibody (2G1) [NBP2-45519] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: ERCC8 Antibody (2G1) [NBP2-45519] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: ERCC8 Antibody (2G1) [NBP2-45519] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI2G1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-70412AF350
Alexa Fluor 405
NBP2-70412AF405
Alexa Fluor 488
NBP2-70412AF488
Alexa Fluor 532
NBP2-70412AF532
Alexa Fluor 594
NBP2-70412AF594
Alexa Fluor 647
NBP2-70412AF647
Alexa Fluor 700
NBP2-70412AF700
Alexa Fluor 750
NBP2-70412AF750
Allophycocyanin
NBP2-70412APC
Biotin
NBP2-70412B
DyLight 350
NBP2-70412UV
DyLight 405
NBP2-70412V
DyLight 488
NBP2-70412G
DyLight 550
NBP2-70412R
DyLight 594
NBP2-70412DL594
DyLight 650
NBP2-70412C
DyLight 680
NBP2-70412FR
DyLight 755
NBP2-70412IR
FITC
NBP2-70412F
HRP
NBP2-70412H
Janelia Fluor 549
NBP2-70412JF549
Janelia Fluor 646
NBP2-70412JF646
PE
NBP2-70412PE
PerCP
NBP2-70412PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-70412
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ERCC8 Antibody (OTI2G1) Summary

Immunogen
Full length human recombinant protein of human ERCC8(NP_001007235) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ERCC8
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
43.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ERCC8 Antibody (OTI2G1)

  • CKN1DNA excision repair protein ERCC-8
  • Cockayne syndrome 1 (classical)
  • Cockayne syndrome WD repeat protein CSA
  • CSACockayne syndrome WD-repeat protein CSA
  • excision repair cross-complementing rodent repair deficiency, complementationgroup 8

Background

ERCC8 encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ERCC8 Antibody
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NBP2-45519
ERCC8 Antibody (OTI2G1)
Species: Hu, Mu, Rt
Applications: WB, IHC

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Bioinformatics

Gene Symbol ERCC8