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EphB1 Antibody (5F10A4) - BSA Free

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Western Blot: EphB1 Antibody (5F10A4) [NBP1-28887] - Analysis using EhpB1 mouse mAb against MDA-MB-468 (1), MDA-MB-453 (2), MCF-7 (3), T47D (4) and SKBR-3 (5) cell lysate.
Immunohistochemistry-Paraffin: EphB1 Antibody (5F10A4) [NBP1-28887] - Analysis of human lung cancer (left) and colon cancer (right) showing cytoplasmic localization with DAB staining using EphB1 mouse mAb.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
5F10A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

EphB1 Antibody (5F10A4) - BSA Free Summary

Immunogen
Purified recombinant fragment of EphB1 (aa19-133) expressed in E. Coli.
Specificity
Specific for EPH Receptor B1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EPHB1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for EphB1 Antibody (5F10A4) - BSA Free

  • Cek6
  • EC 2.7.10
  • EC 2.7.10.1
  • EK6
  • Elk
  • EPH receptor B1
  • eph tyrosine kinase 2
  • EphB1
  • EPH-like kinase 6
  • ephrin type-B receptor 1
  • EPHT2FLJ37986
  • Hek6
  • Net
  • NETHek6
  • soluble EPHB1 variant 1
  • Tyrosine-protein kinase receptor EPH-2

Background

EPH receptor B1. It is a receptor for ephrin-B family members. Ephrin receptors and their ligands, the ephrins, medate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EPHB1
Uniprot