Western Blot: HUWE1 Antibody [NB100-652] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 antibody used for WB at 0.1 ...read more
Immunohistochemistry-Paraffin: HUWE1 Antibody [NB100-652] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiLasu1/Ureb1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.
Immunoprecipitation: HUWE1 Antibody [NB100-652] - Detection of human Lasu1/Ureb1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared ...read more
Viral propagation is required for the induction of cell death through suppression of MCL1 expression. (E) Interferon treatment attenuates JEV-induced suppression of MCL1 expression Huh7 cells infected with JEV (MOI = 5) ...read more
The immunogen this antibody was made to, maps to a region between residues 2250 and 2300 of human Upstream Regulatory Element Binding Protein 1 using the numbering given in entry NP_113584.3 (GeneID 10075).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HUWE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
ICC/IF reactivity reported in (PMID: 27146073). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
481 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Mouse reactivity reported in scientific literature (PMID:31959916).
Packaging, Storage & Formulations
Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified
Alternate Names for HUWE1 Antibody
arf-bp1
EC 6.3.2
EC 6.3.2.6
HECT, UBA and WWE domain containing 1
HECTH9
HSPC272
KIAA0312MULE
Mcl-1 ubiquitin ligase E3
Mule
UREB1UBA and WWE domain-containing protein 1
URE-binding protein 1
Background
ARFBP1 is a novel E3 ubiquitin ligase. It interacts with p19ARF and its ligase activity is inhibited by this interaction.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for HUWE1 Antibody (NB100-652). (Showing 1 - 1 of 1 FAQs).
Can you tell me what percentage of gel was used for the Western Blot and what the transfer time and conditions were for product NB100-652.
This product is tested by a collaborator so I do not have the specifics for this antibody in particular at the moment. I have consulted our lab and for a large protein like this (around 480kDa) we recommend either a 5% or 3-8% gradient polyacrylamide gel. Additionally, we recommend that you boil your samples for 10 minutes at 68 degrees and use your standard transfer conditions. If you need a ladder for that high of a molecular weight, we use HiMark from Invitrogen.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
=
÷
Review this Product
Be the first to review our HUWE1 Antibody and receive a gift card or discount.