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EOMES Antibody (CL15426) [Unconjugated]

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Staining of rat cerebellum shows strong nuclear positivity in cells in the granular layer.
Staining of rat cerebellum shows strong nuclear positivity in cells in the granular layer.
Staining of human liver shows no positivity in hepatocytes as expected.
Staining of mouse embryo, E14 shows strong nuclear positivity in the developing cerebral cortex.
Staining of human kidney shows no positivity in cells in tubules as expected.
Orthogonal Strategies: Analysis in human tonsil and kidney tissues using NBP3-43668 antibody. Corresponding EOMES RNA-seq data are presented for the same tissues.
Staining of human tonsil shows moderate to strong nuclear positivity in non-germinal center cells.
Staining of human cerebellum shows moderate to strong nuclear positivity in a subset of cells in granular layer.

Product Details

Summary
Reactivity Hu, Mu, Rt, Mu, RtSpecies Glossary
Applications IHC
Clone
CL15426
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
       

Orthogonal Strategies

  

Order Details

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EOMES Antibody (CL15426) [Unconjugated] Summary

Immunogen
This antibody was developed against a recombinant protein of O95936. Actual immunogen sequence is proprietary.
Predicted Species
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EOMES
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200-1:500
  • Immunohistochemistry-Paraffin 1:200-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for EOMES Antibody (CL15426) [Unconjugated]

  • EOMES
  • eomesodermin (Xenopus laevis) homolog
  • Eomesodermin
  • t box, brain, 2
  • T-box brain protein 2
  • T-box brain2
  • TBR2
  • TBR-2
  • TBR2eomesodermin homolog
  • T-brain-2

Background

EOMES encodes a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar gene disrupted in mice is shown to be essential during trophoblast development and gastrulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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EOMES Antibody
(CL15426) [Unconjugat
NBP3-43668
EOMES Antibody (CL15426) [Unconjugated]
Species: Hu, Mu, Rt, Mu, Rt
Applications: IHC

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Bioinformatics

Gene Symbol EOMES