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eNOS Antibody [Unconjugated]

 (12 Publications)
 
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Western blot shows lysates of HUVEC human umbilical vein endothlial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eNOS Affinity-purified Polyclonal Antibody (Catalog # AF950) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eNOS at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows lysates of HUVEC human umbilical vein endothlial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human eNOS Affinity-purified Polyclonal Antibody (Catalog # AF950) followed by ...read more
eNOS was immunoprecipitated from lysates (1 x 106cells) of HUVEC human umbilical vein endothelial cells following incubation with 0.3 µg Goat Anti-Human eNOS Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for eNOS at approximately 139 kDa (as indicated) using 10 µg/mL of Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF950
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF950-SP
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eNOS Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human eNOS
Specificity
Detects human eNOS in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NOS3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 0.3 ug/10^6 cells
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF950 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for eNOS Antibody [Unconjugated]

  • Constitutive NOS
  • EC 1.14.13.39
  • ECNOS
  • Endothelial NOS
  • endothelial
  • eNOS
  • eNOScNOS
  • nitric oxide synthase 3 (endothelial cell)
  • NOS type III
  • NOS3
  • NOSIII

Background

Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is an enzyme encoded by the NOS3 gene. Endothelial NOS generates nitric oxide in blood vessels and is involved with regulating vascular tone by inhibiting smooth muscle contraction and platelet aggregation. A constitutive calcium dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of the Golgi apparatus within cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for eNOS Antibody (AF950)(12)

We have publications tested in 3 confirmed species: Human, Guinea Pig, Porcine.

We have publications tested in 6 applications: ICC, IHC-Fr, IHC-P, Immunocytochemistry, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(1)
IHC-Fr
(1)
IHC-P
(1)
Immunocytochemistry
(1)
Immunoprecipitation
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(7)
Guinea Pig
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using AF950 Applications Species
Festa, J;Hussain, A;Al-Hareth, Z;Bailey, SJ;Singh, H;Da Boit, M; Phenolic Metabolites Protocatechuic Acid and Vanillic Acid Improve Nitric Oxide Bioavailability via the Akt-eNOS Pathway in Response to TNF-? Induced Oxidative Stress and Inflammation in Endothelial Cells Metabolites 2024-11-11 [PMID: 39590849] (Western Blot, Human) Western Blot Human
Festa J, Hussain A, Hackney A et al. Elderberry extract improves molecular markers of endothelial dysfunction linked to atherosclerosis Food Science & Nutrition 2023-07-01 [PMID: 37457144]
Hussong, SA;Banh, AQ;Van Skike, CE;Dorigatti, AO;Hernandez, SF;Hart, MJ;Ferran, B;Makhlouf, H;Gaczynska, M;Osmulski, PA;McAllen, SA;Dineley, KT;Ungvari, Z;Perez, VI;Kayed, R;Galvan, V; Soluble pathogenic tau enters brain vascular endothelial cells and drives cellular senescence and brain microvascular dysfunction in a mouse model of tauopathy Nature communications 2023-04-25 [PMID: 37185259] (Immunocytochemistry, Human) Immunocytochemistry Human
Z Wang, F Wang, X Kong, X Gao, Y Gu, J Zhang Oscillatory Shear Stress Induces Oxidative Stress via TLR4 Activation in Endothelial Cells Mediators Inflamm., 2019-06-17;2019(0):7162976. 2019-06-17 [PMID: 31316302] (IHC-Fr, Western Blot, Human) IHC-Fr, Western Blot Human
Jonathan Noonan, Gianluca Grassia, Neil MacRitchie, Paul Garside, Tomasz J. Guzik, Angela C. Bradshaw et al. A Novel Triple-Cell Two-Dimensional Model to Study Immune-Vascular Interplay in Atherosclerosis Frontiers in Immunology 2019-04-24 [PMID: 31068936]
P Guerby, A Swiader, N Augé, O Parant, C Vayssière, K Uchida, R Salvayre, A Negre-Salv High glutathionylation of placental endothelial nitric oxide synthase in preeclampsia Redox Biol, 2019-01-26;22(0):101126. 2019-01-26 [PMID: 30738311] (Immunoprecipitation, Human) Immunoprecipitation Human
Bing Liu, Lili Xu, Xinming Yu, Wei Li, Xiaozhi Sun, Shun Xiao, Mingjin Guo, Haofu Wang Protective effect of KLF15 on vascular endothelial dysfunction induced by TNF??. Molecular Medicine Reports, 2018-06-20;0(0):1791-3004. 2018-06-20 [PMID: 29956764] (Western Blot, Human) Western Blot Human
Y Liu, Y Huang, Y Xu, P Qu, M Wang Memantine protects against ischemia/reperfusion-induced brain endothelial permeability IUBMB Life, 2018-03-15;0(0):. 2018-03-15 [PMID: 29542225] (Western Blot, Human) Western Blot Human
Travis W. Hein, Robert H. Rosa, Yi Ren, Wenjuan Xu, Lih Kuo VEGF Receptor-2–Linked PI3K/Calpain/SIRT1 Activation Mediates Retinal Arteriolar Dilations to VEGF and Shear Stress Investigative Opthalmology & Visual Science 2015-08-19 [PMID: 26284543]
Kawai Y, Yokoyama Y, Kaidoh M, Ohhashi T Shear stress-induced ATP-mediated endothelial constitutive nitric oxide synthase expression in human lymphatic endothelial cells. Am. J. Physiol., Cell Physiol., 2009-12-30;298(3):C647-55. 2009-12-30 [PMID: 20042732] (ICC, Human) ICC Human
Show All 12 Publications.

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Bioinformatics

Gene Symbol NOS3