Western blot shows lysates of human brain, mouse brain tissue, and BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Human/Mouse Enolase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog ...read more
Western blot shows lysates of rat brain tissue, rat cerebellum tissue, and rat olfactroy bulb tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse Enolase 2/Neuron-specific Enolase Antigen ...read more
Enolase 2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human/Mouse Enolase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) at 10 µg/mL overnight at 4 °C. ...read more
Enolase 2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human/Mouse Enolase 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5169) at 10 µg/mL overnight at 4 °C. ...read more
Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for Enolase 2/Neuron‑specific Enolase at approximately 50 kDa (as indicated) using 10 ...read more
E. coli-derived recombinant human Enolase 2/Neuron-specific Enolase Met1-Leu434 Accession # P09104
Specificity
Detects human and mouse Enolase 2/Neuron-specific Enolase in direct ELISAs. Detects human, mouse, and rat Enolase 2/Neuron-specific Enolase in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ENO2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Enolase 2/Neuron-specific Enolase Antibody [Unconjugated]
2-phospho-D-glycerate hydrolyase
2-phospho-D-glycerate hydro-lyase
EC 4.2.1.11
ENO2
enolase 2 (gamma, neuronal)
Enolase 2
gamma-Enolase
Neural enolase
neuron specific gamma enolase
neurone-specific enolase
Neuronspecific Enolase
Neuron-specific enolase
NSE
Background
Enolase 2 (2-phospho-D-glycerate hydrolyase; also neural enolase and gamma -enolase) is a 46 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer, or heterodimer with alpha -enolase. Full-length human Enolase 2 is 99% aa identical to both mouse and canine Enolase 2. It shares 83% aa identity with human enolases # 1 and # 3.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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