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Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC, MA, CyTOF-ready
Clone
ENO2/1462
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700

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Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [Alexa Fluor® 700] Summary

Immunogen
A synthetic peptide of human Enolase 2/Neuron-specific Enolase (around aa416-433) (exact sequence is proprietary) (Uniprot: P09104)
Localization
Cytoplasmic
Marker
Neuroendocrine Marker
Specificity
The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ENO2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Protein Array
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [Alexa Fluor® 700]

  • 2-phospho-D-glycerate hydrolyase
  • 2-phospho-D-glycerate hydro-lyase
  • EC 4.2.1.11
  • ENO2
  • enolase 2 (gamma, neuronal)
  • Enolase 2
  • gamma-Enolase
  • Neural enolase
  • neuron specific gamma enolase
  • neurone-specific enolase
  • Neuronspecific Enolase
  • Neuron-specific enolase
  • NSE

Background

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ENO2